Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA.
Department of Pharmaceutical Sciences, School of Pharmacy, Binghamton University, Binghamton, NY, USA.
Sci Rep. 2017 Jul 10;7(1):5005. doi: 10.1038/s41598-017-05117-2.
Despite their well-known function in maintaining normal cell physiology, how inorganic elements are relevant to cellular pluripotency and differentiation in human pluripotent stem cells (hPSCs) has yet to be systematically explored. Using total reflection X-ray fluorescence (TXRF) spectrometry and inductively coupled plasma mass spectrometry (ICP-MS), we analyzed the inorganic components of human cells with isogenic backgrounds in distinct states of cellular pluripotency. The elemental profiles revealed that the potassium content of human cells significantly differs when their cellular pluripotency changes. Pharmacological treatment that alters cell membrane permeability to potassium affected the maintenance and establishment of cellular pluripotency via multiple mechanisms in bona fide hPSCs and reprogrammed cells. Collectively, we report that potassium is a pluripotency-associated inorganic element in human cells and provide novel insights into the manipulation of cellular pluripotency in hPSCs by regulating intracellular potassium.
尽管无机元素在维持正常细胞生理功能方面是众所周知的，但它们如何与人类多能干细胞（hPSCs）中的细胞多能性和分化相关，尚未得到系统的探索。本研究使用全反射 X 射线荧光（TXRF）光谱法和电感耦合等离子体质谱法（ICP-MS），分析了具有不同细胞多能性状态的同基因背景的人类细胞中的无机成分。元素图谱显示，当人类细胞的细胞多能性发生变化时，其钾含量有显著差异。改变细胞膜对钾通透性的药物处理通过多种机制影响真正的 hPSCs 和重编程细胞的细胞多能性的维持和建立。总的来说，我们报道钾是人类细胞中与多能性相关的无机元素，并为通过调节细胞内钾来操纵 hPSCs 中的细胞多能性提供了新的见解。
