Department of Plant Physiology, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, Poland.
Plant Sci. 2012 Feb;183:143-8. doi: 10.1016/j.plantsci.2011.08.004. Epub 2011 Aug 24.
Strictly controlled and coordinated induction of CBF regulon (a set of genes regulated by CBF proteins) promotes plant freezing tolerance. CBFs regulate the expression of COR genes that confer freezing tolerance. COR14b in barley is one of the effector genes which seems to be important in resistance to combined freezing and photoinhibition of photosynthesis. LOS2 represses the transcription of STS/ZAT10 (a negative regulator of CBF-target genes) and thus acts as a positive regulator of COR genes. In Arabidopis, low temperature induction of CBFs was reported to be gated by the circadian clock. Moreover, light-quality signals have been shown to regulate some plants' freezing tolerance genes. The aim of our study was to determine the effects of combined treatment with light and cold on the transcript levels of CBF6, FpCor14b and LOS2 genes in Festuca pratensis. We have demonstrated that the regulation of CBF6, FpCor14b and LOS2 induction kinetics in F. pratensis occurs through the interaction of temperature and light with time of day during low-temperature shift. The FpCOR14b transcript level was shown to be up-regulated by increasing light intensity. It was also proved that light quality strongly regulates CBF6, FpCor14b and LOS2 transcripts induction kinetics at low temperatures.
严格控制和协调 CBF 调控因子（一组受 CBF 蛋白调控的基因）的诱导可促进植物的抗冻性。CBF 调节 COR 基因的表达，赋予植物抗冻性。大麦中的 COR14b 是一种效应基因，似乎在抵抗冻融和光合作用光抑制方面很重要。LOS2 抑制 STS/ZAT10（CBF 靶基因的负调节剂）的转录，因此作为 COR 基因的正调节剂起作用。在拟南芥中，CBF 的低温诱导被生物钟调控。此外，光质信号已被证明可调节某些植物的抗冻性基因。我们的研究目的是确定光和冷联合处理对羊茅 CBF6、FpCor14b 和 LOS2 基因转录水平的影响。我们已经证明，在低温转换过程中，通过温度和光与一天中时间的相互作用，调节羊茅 CBF6、FpCor14b 和 LOS2 诱导动力学。研究表明，增加光照强度可上调 FpCOR14b 转录本水平。还证明了在低温下，光质强烈调节 CBF6、FpCor14b 和 LOS2 转录本的诱导动力学。
