Liu Lihong, Chen Ying, Zhang Yulin, Wang Fang, Chen Zilin
Institute of Pharmaceutical Analysis, College of Pharmacy, Wuhan University, Wuhan 430072, China.
Biomed Chromatogr. 2011 Aug;25(8):938-42. doi: 10.1002/bmc.1550. Epub 2010 Dec 10.
A novel method was developed for the simultaneous determination of kynurenine and tryptophan by high-performance liquid chromatography with electrochemical detection at multi-wall carbon nanotube (MWCNT)-modified glassy carbon electrode. The separation and detection conditions were optimized. The typical HPLC experiments were conducted by using a reversed-phase ODS column with a mobile phase consisting of stock acetate buffer (pH 5)-methanol (4:1, v/v) using an isocratic elution at the flow rate of 1.0 mL/min. The obtained LODs for kynurenine and tryptophane were 0.5 and 0.4 µmol/L, respectively. The analytical method for human plasma samples was validated and confirmed by LC-UV and LC-MS. The recoveries were in the range of 84.8-110%, and the precision was lower than 5.9%.
开发了一种通过在多壁碳纳米管（MWCNT）修饰的玻碳电极上进行电化学检测的高效液相色谱法同时测定犬尿氨酸和色氨酸的新方法。优化了分离和检测条件。典型的高效液相色谱实验是使用反相ODS柱进行的，流动相由储备醋酸盐缓冲液（pH 5）-甲醇（4:1，v/v）组成，采用等度洗脱，流速为1.0 mL/min。犬尿氨酸和色氨酸的检测限分别为0.5和0.4 μmol/L。通过LC-UV和LC-MS对人血浆样品的分析方法进行了验证和确认。回收率在84.8-110%范围内，精密度低于5.9%。
