León Fernández Olga Sonia, Pantoja Marelis, Díaz Soto María Teresa, Dranguet Jaqueline, García Insua Martina, Viebhan-Hánsler Renata, Menéndez Cepero Silvia, Calunga Fernández José L
Department of Pharmacology/Toxicology from Institute of Pharmacy and Food Sciences, University of Havana, Cuba.
Neurol Res. 2012 Jan;34(1):59-67. doi: 10.1179/1743132811Y.0000000060.
INTRODUCTION/OBJECTIVES: Although inflammation in disc hernia (DH) has been recognized and it is a well-known process mediated by loss of the cellular redox balance, only a few studies about the impact of chronic oxidative stress on this neurological disorder have been made. Ozone therapy has been widely used with clinical efficacy in DH. This work aimed at characterizing the systemic redox status of patients with low back pain and neck pain as well as studying if ozone oxidative post-conditioning modified the pathological oxidative stress and protected against oxidative protein damage and if there is any relationship between oxidative changes and pain in both DH.
Redox status of 33 patients with diagnosis of DH by computerized axial tomography, nuclear magnetic resonance, and clinical evaluations was studied. Ozone was administered by paravertebral way. After ozone treatment, plasmatic levels of antioxidant/pro-oxidant markers, pain, and life quality disability parameters were evaluated.
One hundred percent of patients showed a severe oxidative stress. Major changes in superoxide dismutase activity, total hydroperoxides, advanced oxidation protein products, fructolysine content, and malondialdehyde were observed. After ozone oxidative post-conditioning, there was a re-establishment of patients' cellular redox balance as well as a decrease in pain in both DH. A relationship between indicators of oxidative protein damage and pain was demonstrated.
Ozone therapy protected against oxidation of proteins and reduced the pain. Relationship between markers of oxidative protein damage, disability parameters, and pain suggests the role of oxidative stress in the pathological processes involved in DH.
引言/目的：尽管椎间盘突出症（DH）中的炎症已得到认可，且这是一个由细胞氧化还原平衡丧失介导的众所周知的过程，但关于慢性氧化应激对这种神经疾病影响的研究却很少。臭氧疗法已在DH中广泛应用并具有临床疗效。这项工作旨在表征下腰痛和颈痛患者的全身氧化还原状态，以及研究臭氧氧化后处理是否能改善病理性氧化应激并防止氧化蛋白损伤，以及在两种DH中氧化变化与疼痛之间是否存在任何关系。
对33例经计算机断层扫描、核磁共振和临床评估诊断为DH的患者的氧化还原状态进行了研究。通过椎旁途径给予臭氧。臭氧治疗后，评估抗氧化/促氧化标志物的血浆水平、疼痛和生活质量残疾参数。
100%的患者表现出严重的氧化应激。观察到超氧化物歧化酶活性、总氢过氧化物、晚期氧化蛋白产物、果糖赖氨酸含量和丙二醛有重大变化。臭氧氧化后处理后，患者的细胞氧化还原平衡得以重建，两种DH的疼痛均有所减轻。氧化蛋白损伤指标与疼痛之间的关系得到了证实。
臭氧疗法可防止蛋白质氧化并减轻疼痛。氧化蛋白损伤标志物、残疾参数和疼痛之间的关系表明氧化应激在DH所涉及的病理过程中的作用。
