School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
Biosens Bioelectron. 2012 Feb 15;32(1):183-7. doi: 10.1016/j.bios.2011.12.001. Epub 2011 Dec 9.
In order to develop facile, fast and sensitive detection methods for endocrine-disrupting chemicals (EDCs), we described a sensitive biosensing system involving magnetic relaxation switch, based on the assembly of cross-linked superparamagnetic iron oxide (CLIO) nanoparticles induced by the antigen-antibody biorecognition. The design of smart CLIO-based superparamagnetic iron oxide nanoparticles and antigen-OVA was described for the detection of bisphenol A [2,2-bis (4-hydroxyphenol) propane (BPA)]. The addition of BPA to the rapid magnetic relaxation switch immunosensor led to transverse relaxation time (T2) shortening compared to a blank control as shown by NMR relaxometry measurements. This process was also applied to the rapid and facile determination of concentrations of BPA in drinking water (tap water). Good linearity for all calibration curves was obtained, and the limit of detection (LOD) for BPA was 0.4 ng/mL in tap water.
为了开发用于检测内分泌干扰化学物质（EDCs）的简便、快速和灵敏的检测方法，我们描述了一种基于免疫识别的交联超顺磁性氧化铁（CLIO）纳米粒子组装的灵敏生物传感系统。设计了基于智能 CLIO 的超顺磁性氧化铁纳米粒子和抗原-OVA，用于检测双酚 A [2,2-双（4-羟基苯基）丙烷（BPA）]。与空白对照相比，向快速磁共振弛豫开关免疫传感器中加入 BPA 会导致横向弛豫时间（T2）缩短，如 NMR 弛豫测量所示。该过程还应用于快速简便地测定饮用水（自来水）中的 BPA 浓度。所有校准曲线均呈现良好的线性，自来水中 BPA 的检测限（LOD）为 0.4ng/mL。
