State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, PR China.
State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, PR China.
Biosens Bioelectron. 2017 Jun 15;92:21-25. doi: 10.1016/j.bios.2017.01.041. Epub 2017 Jan 21.
In this study, we designed and fabricated an electrochemical impedance aptasensor based on Au nanoparticles (Au-NPs) coated boron-doped diamond (BDD) modified with aptamers, and 6-mercapto-1-hexanol (MCH) for the detection of bisphenol A (BPA). The constructed BPA aptasensor exhibits good linearity from 1.0×10 to 1.0×10molL. The detection limitation of 7.2×10molL was achieved, which can be attributed to the synergistic effect of combining BDD with Au-NPs, aptamers, and MCH. The examine results of BPA traces in Tris-HCl buffer and in milk, UV spectra of aptamer/BPA and interference test revealed that the novel aptasensors are of high sensitivity, specificity, stability and repeatability, which could be promising in practical applications.
在这项研究中，我们设计并制造了一种基于金纳米粒子（Au-NPs）的电化学阻抗适体传感器，该传感器涂有硼掺杂金刚石（BDD），并修饰了适体和 6-巯基-1-己醇（MCH），用于检测双酚 A（BPA）。所构建的 BPA 适体传感器在 1.0×10 到 1.0×10molL 范围内表现出良好的线性。检测限达到 7.2×10molL，可以归因于 BDD 与 Au-NPs、适体和 MCH 的协同作用。在 Tris-HCl 缓冲液和牛奶中检测 BPA 痕迹的结果、适体/BPA 的紫外光谱和干扰测试表明，新型适体传感器具有高灵敏度、特异性、稳定性和可重复性，在实际应用中具有广阔的应用前景。
