Thornbury D W, Patterson C A, Dessens J T, Pirone T P
Department of Plant Pathology, University of Kentucky, Lexington 40546.
Virology. 1990 Oct;178(2):573-8. doi: 10.1016/0042-6822(90)90356-v.
Potato virus C (PVC), a non-aphid transmissible strain of potato virus Y (PVY), was found to code for a protein (PVC-HC) which is similar in molecular weight and immunological reactivity to the helper component protein of PVY (PVY-HC). PVC-HC, however, was inactive with respect to its ability to effect aphid transmission of either PVC or PVY. The 5'-terminal 2.7-kb regions of PVC and PVY were sequenced. Within the HC region there was 92% nucleotide homology between the two strains; comparison of the derived amino acid sequences revealed 24 amino acid differences. Comparison of the PVC-HC sequence with that of five potyviruses revealed 2 amino acid changes which were specific to PVC-HC. These amino acids are prime targets for mutational analysis of HC activity.
马铃薯病毒C（PVC）是马铃薯Y病毒（PVY）的一种非蚜虫传播株系，被发现编码一种蛋白质（PVC-HC），其分子量和免疫反应性与PVY的辅助成分蛋白（PVY-HC）相似。然而，PVC-HC在影响PVC或PVY的蚜虫传播能力方面是无活性的。对PVC和PVY的5'-末端2.7kb区域进行了测序。在HC区域内，两个株系之间有92%的核苷酸同源性；对推导的氨基酸序列进行比较，发现有24个氨基酸差异。将PVC-HC序列与五种马铃薯Y病毒的序列进行比较，发现有2个氨基酸变化是PVC-HC特有的。这些氨基酸是HC活性突变分析的主要靶点。
