Nakashima H, Sako N, Hori K
Faculty of Agriculture, Saga University, Japan.
Arch Virol. 1993;131(1-2):17-27. doi: 10.1007/BF01379077.
We have compared nucleotide sequences of the helper component-proteinase (HC-Pro) coding region of aphid transmissible (isolate 1) and non-transmissible (isolate 31) isolates of turnip mosaic virus (TuMV). HC-Pro coding regions of both TuMV isolates 1 and 31 were 1,374 nucleotide long. The nucleotide sequence homology between these isolates was 93.5%, with 89 nucleotides substitution. The nucleotides of HC-Pro regions of two isolates of TuMV genomes encoded 458 amino acids of M(r) 51,746 (isolate 1) and M(r) 51,764 (isolate 31). The deduced amino acid sequence homology between these isolates was 98.7% with six different amino acids. These amino acids appeared to regulate the activity of HC-Pro needed for aphid transmissibility of TuMV.
我们比较了芜菁花叶病毒（TuMV）的蚜虫可传播分离株（分离株1）和不可传播分离株（分离株31）的辅助成分蛋白酶（HC-Pro）编码区的核苷酸序列。TuMV分离株1和31的HC-Pro编码区均为1374个核苷酸长。这些分离株之间的核苷酸序列同源性为93.5%，有89个核苷酸替换。TuMV基因组两个分离株的HC-Pro区核苷酸编码了458个氨基酸，其分子量分别为51746（分离株1）和51764（分离株31）。这些分离株之间推导的氨基酸序列同源性为98.7%，有六个不同的氨基酸。这些氨基酸似乎调节了TuMV蚜虫传播所需的HC-Pro活性。
