Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900 Rio de Janeiro, Brazil.
J Microbiol Methods. 2012 Feb;88(2):255-62. doi: 10.1016/j.mimet.2011.12.004. Epub 2011 Dec 16.
Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficacy of sterilizing and disinfectant products, used for semi critical medical devices, against the Mycobacterium bovis BCG Moreau and Mycobacterium abscessus subsp. bolletii INCQS 00594 strains by using the Confirmative in vitro Test for Determining Tuberculocidal Activity of Disinfectants recommended by the Association of Official Analytical Chemists. These changes have caused additional costs and increased problems for importation of enrichment products at national laboratories where disinfectant efficacy assay service is performed. Middlebrook ADC Enrichment (ADC) is provided by a unique manufacturer and used in the official protocol. The aim of the present study was to evaluate an alternative in house low-cost enrichment composed of fetal bovine serum and glucose (FBSG) with ADC for performance of disinfectant efficacy assay against mycobacteria. After obtaining the growth curves for M. abscessus ATCC 19977, M. abscessus subsp. bolletii INCQS 00594, Mycobacterium chelonae ATCC 35752, and Mycobacterium fortuitum ATCC 6841 by using ADC enrichment and FBSG in Kirchners and 7H9 culture media. Through statistical analysis via the Kruskal-Wallis test on the evaluation of microorganism growth rate, it was observed that there was no inhibition of RGM growth by any of the enrichments used. These results suggest that low-cost enrichment FBSG may be used as a potential substitute of ADC for composition of media for mycobacterial growth, including in disinfectant tests.
巴西最近对商业消毒剂的立法进行了修改，原因是过去 8 年来，巴西许多州的医院感染迅速增长的分枝杆菌（RGM）的爆发。其中一份文件要求所有制造商提供证据证明用于半关键医疗器械的消毒和消毒剂产品对牛分枝杆菌 BCG Moreau 和脓肿分枝杆菌亚种 bolletii INCQS 00594 菌株的杀菌和消毒效果，方法是使用美国分析化学家协会推荐的确认体外试验来确定消毒剂的杀菌活性。这些变化给国家实验室进口增效剂带来了额外的成本和问题，这些实验室进行消毒剂功效检测服务。Middlebrook ADC 增菌（ADC）由唯一的制造商提供，并且在官方协议中使用。本研究的目的是评估一种替代的低成本国产增菌剂，由胎牛血清和葡萄糖（FBSG）组成，与 ADC 一起用于对抗分枝杆菌的消毒剂功效检测。通过在 Kirchners 和 7H9 培养基中使用 ADC 增菌和 FBSG 获得脓肿分枝杆菌 ATCC 19977、脓肿分枝杆菌亚种 bolletii INCQS 00594、龟分枝杆菌 ATCC 35752 和偶然分枝杆菌 ATCC 6841 的生长曲线。通过对微生物生长率评估的 Kruskal-Wallis 检验进行统计分析，观察到任何增菌剂都没有抑制 RGM 的生长。这些结果表明，低成本的 FBSG 增菌剂可以作为 ADC 的潜在替代品，用于分枝杆菌生长的培养基的组成，包括消毒剂测试。
