Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany.
Vaccine. 2012 Feb 1;30(6):989-94. doi: 10.1016/j.vaccine.2011.12.057. Epub 2011 Dec 23.
In recent years the safety of the yellow fever live vaccine 17D came under scrutiny. The focus was on serious adverse events after vaccinations that resemble a wild type infection with yellow fever and whose reasons are still not known. Also the exact mechanism of attenuation of the vaccine remains unknown to this day. In this context, the standards of safety and surveillance in vaccine production and administration have been discussed. Therein embodied was the demand for improved documentation of the derivation of the seed virus used for yellow fever vaccine production. So far, there was just a historical genealogy available that is based on source area and passage level. However, there is a need for a documentation based on molecular information to get better insights into the mechanisms of pathology. In this work we sequenced the whole genome of different passages of the YFV-17D strain used by Crucell Switzerland AG for vaccine production. Using all other publically available 17D full genome sequences we compared the sequence variance of all vaccine strains and oppose a phylogenetic tree based on full genome sequences to the historical genealogy.
近年来，黄热病活疫苗 17D 的安全性受到了关注。重点是接种后类似于黄热病野生型感染的严重不良反应，其原因仍不清楚。此外，疫苗减毒的确切机制至今仍不清楚。在这方面，讨论了疫苗生产和管理中安全性和监测的标准。其中包括要求更好地记录用于黄热病疫苗生产的种子病毒的衍生。到目前为止，只有一个基于来源区域和传代数的历史谱系。然而，需要基于分子信息的文档记录，以更好地了解发病机制。在这项工作中，我们对 Crucell Switzerland AG 用于疫苗生产的 YFV-17D 株的不同传代数进行了全基因组测序。使用所有其他公开的 17D 全基因组序列，我们比较了所有疫苗株的序列变异，并基于全基因组序列构建了一个系统发育树，与历史谱系相对比。
