Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
J Clin Microbiol. 2018 May 25;56(6). doi: 10.1128/JCM.00323-18. Print 2018 Jun.
Yellow fever (YF) is a reemerging public health threat, with frequent outbreaks prompting large vaccination campaigns in regions of endemicity in Africa and South America. Specific detection of vaccine-related adverse events is resource-intensive, time-consuming, and difficult to achieve during an outbreak. To address this, we have developed a highly transferable rapid yellow fever virus (YFV) vaccine-specific real-time reverse transcription-PCR (RT-PCR) assay that distinguishes vaccine from wild-type lineages. The assay utilizes a specific hydrolysis probe that includes locked nucleic acids to enhance specific discrimination of the YFV17D vaccine strain genome. Promisingly, sensitivity and specificity analyses reveal this assay to be highly specific to vaccine strain(s) when tested on clinical samples and YFV cell culture isolates of global origin. Taken together, our data suggest the utility of this assay for use in laboratories of varied capacity for the identification and differentiation of vaccine-related adverse events from wild-type infections of both African and South American origin.
黄热病（YF）是一种重新出现的公共卫生威胁，在非洲和南美洲的流行地区经常爆发，需要开展大规模疫苗接种活动。针对疫苗相关不良事件的具体检测需要耗费大量资源，既耗时又困难。因此，我们开发了一种高度可转移的快速黄热病病毒（YFV）疫苗特异性实时逆转录聚合酶链反应（RT-PCR）检测方法，可区分疫苗株和野生型病毒株。该检测方法利用一种特异性水解探针，其中包含锁定核酸，以增强对 YFV17D 疫苗株基因组的特异性区分。有希望的是，敏感性和特异性分析表明，当在临床样本和源自全球的 YFV 细胞培养分离物上进行测试时，该检测方法对疫苗株具有高度特异性。总的来说，我们的数据表明，该检测方法可用于不同能力的实验室，用于鉴定和区分源自非洲和南美洲的野生型感染与疫苗相关的不良事件。
