Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Gaja-Dong 900, Jinju, Gyeongnam 660701, Republic of Korea.
Anaerobe. 2012 Feb;18(1):96-102. doi: 10.1016/j.anaerobe.2011.12.015. Epub 2011 Dec 19.
Three Weissella confusa and five Weissella cibaria strains were previously isolated from human faeces and their potential as probiotics was examined in this work. Resistance to low pHs (pH 2.0 and 3.0) and 0.3% bile salt were examined. Enzyme activities, susceptibilities to heat treatment and various antibiotics, and adhesion capacities to Caco-2 cells were also examined. All Weissella strains were killed when exposed to pH 2.0 for 2 h but survived at pH 3.0 with different survival ratios. W. confusa 31 survived best (20.2%) and W. confusa 31 was also quite resistant against 0.3% bile salt (128.8%). All strains except one grew well at temperature between 15 and 45 °C and all strains grew in the presence of 6.5% NaCl. W. confusa 20 showed the highest β-galactosidase activity (527.3 ± 23.66 unit/mg protein) and W. cibaria 31 had the highest β-glucosidase activity (115.12 ± 5.3 unit/mg protein) in MRS broth. All strains adhered to Caco-2 cells better than Lactobacillus rhamnosus GG and W. confusa 20 was the best adhesive strain (85 CFU/cell). These results show that some strains such as W. confusa 31 and W. confusa 20 are fully qualified as probiotics and deserve further application studies.
先前从人体粪便中分离出三株魏斯氏菌属混淆亚种和五株魏斯氏菌属栖粪亚种，并在本研究中对其作为益生菌的潜力进行了评估。考察了它们对低 pH 值（pH 2.0 和 3.0）和 0.3%胆汁盐的抗性。还检测了酶活性、对热处理和各种抗生素的敏感性以及对 Caco-2 细胞的黏附能力。所有魏斯氏菌菌株在暴露于 pH 2.0 2 小时后均被杀死，但在 pH 3.0 下以不同的存活率存活。魏斯氏菌属混淆亚种 31 存活最好（20.2%），且对 0.3%胆汁盐也具有较强的抗性（128.8%）。除一株外，所有菌株在 15-45°C 之间的温度下均能良好生长，所有菌株在 6.5%NaCl 存在下均能生长。魏斯氏菌属混淆亚种 20 在 MRS 肉汤中表现出最高的β-半乳糖苷酶活性（527.3 ± 23.66 单位/mg 蛋白），魏斯氏菌属栖粪亚种 31 具有最高的β-葡萄糖苷酶活性（115.12 ± 5.3 单位/mg 蛋白）。所有菌株对 Caco-2 细胞的黏附能力均优于鼠李糖乳杆菌 GG，其中魏斯氏菌属混淆亚种 20 是最佳黏附菌株（85 CFU/细胞）。这些结果表明，一些菌株，如魏斯氏菌属混淆亚种 31 和魏斯氏菌属栖粪亚种 20，完全具备益生菌的资格，值得进一步进行应用研究。
