Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh, India.
Antonie Van Leeuwenhoek. 2012 May;101(4):733-42. doi: 10.1007/s10482-011-9687-6. Epub 2011 Dec 27.
Four yeast strains (APSS 805, APSS 806, APSS 815 and AP-18) belonging to a novel Torulaspora species were isolated from coal mine soils of Singareni in Andhra Pradesh state, India. Another strain (PBA-22) was isolated from agricultural field soil from Gujarat state, India. The vegetative cells of all these strains were round, haploid and produced asci by conjugation between independent cells or mother cell and bud, with rough ascospores, suggesting their possible relation to ascomycetous yeast genus Torulaspora. Phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and Internal Transcribed Spacer (ITS) regions revealed that, among the five strains, three viz. APSS 805, APSS 806 and APSS 815 have identical sequences. The other two strains (AP-18 and PBA-22) differed from the other three strains in less than 1% nucleotide substitutions in the combined D1/D2 domain and ITS sequences, indicating that all of them (five strains) may belong to the same species. These five strains were closely related to Torulaspora globosa, but showed more than 3-7% sequence divergence from T. globosa and all other species in the genus Torulaspora in the combined sequence analysis of D1/D2 domain and ITS region of rRNA gene. In addition, these strains also showed distinct microsatellite finger-printing pattern from related species and differed in several physiological responses suggesting that these strains belong to a novel species of Torulaspora. We propose to name these strains as Torulaspora indica sp. nov., and designate APSS 805(T) = MTCC 9772 (T) = CBS 12408 (T) as the type strain of this novel species. The Mycobank number of the novel species is MB 563738.
从印度安得拉邦 Singareni 煤矿土壤中分离到属于新型毕赤酵母属的 4 株酵母菌株（APSS805、APSS806、APSS815 和 AP-18）。另一株（PBA-22）是从印度古吉拉特邦农业土壤中分离出来的。所有这些菌株的营养细胞均为圆形，单倍体，通过独立细胞或母细胞与芽之间的配合形成子囊，产生粗糙的子囊孢子，表明它们可能与子囊菌属酵母属毕赤酵母有关。大亚基（LSU）rRNA 基因的 D1/D2 结构域和内部转录间隔区（ITS）区域的系统发育分析表明，在这 5 株菌株中，APSS805、APSS806 和 APSS815 具有相同的序列。另外两株（AP-18 和 PBA-22）在 D1/D2 结构域和 ITS 序列的联合序列中与其他三株的核苷酸替换差异小于 1%，表明它们（五株）可能属于同一物种。这五株菌与 T. globosa 密切相关，但在 D1/D2 结构域和 ITS 区联合 rRNA 基因序列分析中与 T. globosa 和该属的所有其他种的序列差异超过 3-7%。此外，这些菌株还表现出与相关种不同的微卫星指纹图谱，并在几个生理反应中表现出差异，表明这些菌株属于新型毕赤酵母属。我们建议将这些菌株命名为新型毕赤酵母属印度种，指定 APSS805（T）=MTCC9772（T）=CBS12408（T）为该新型种的模式株。该新型种的 MycoBank 编号为 MB563738。
