Abdalla Amel, Hamid Mohamed E
Animal Health Department, General Directorate of the Animal Health and Epizootic Disease Control, Federal Ministry of the Animal Resources and Fisheries, Khartoum, Sudan.
Trop Anim Health Prod. 2012 Aug;44(6):1151-5. doi: 10.1007/s11250-011-0051-7. Epub 2011 Dec 27.
The objective of the present study was to detect brucellosis in suspected dairy cattle in Khartoum State, Sudan using the conventional serological tests and tests done on milk in comparison to a PCR-based technique. Milk and blood samples collected simultaneously from suspected brucellosis cows (n = 147) in 12 different dairy farms around Khartoum State were used in the study. Overall, 54 (36.7%) of the total milk samples were positive according to the milk ring test (MRT), while 29 (19.7%) of the serum samples were positive according to the Rose Bengal test (RBT); microscopy on modified Ziehl-Neelsen-stained slides detected 13.6% of the cases, and recovery of Brucella species on both Brucella medium and tryptic soya agar was 7.5%. Thirty-three (22.4%) samples were found positive on PCR-amplified IS711 which were then taken as positive brucellosis cases. The differences of RBT and PCR-IS711 from MRT were highly significant (P < 0.05). MRT detected more cases of bovine brucellosis compared to RBT, PCR, microscopy, and culture. MRT is recommended as a noninvasive test compared to RBT, and it is less expensive compared to PCR and culture.
本研究的目的是使用传统血清学检测方法以及对牛奶进行的检测，并与基于聚合酶链反应（PCR）的技术相比较，来检测苏丹喀土穆州疑似患布鲁氏菌病的奶牛。本研究使用了从喀土穆州周边12个不同奶牛场的疑似布鲁氏菌病奶牛（n = 147）身上同时采集的牛奶和血液样本。总体而言，根据乳环试验（MRT），54份（36.7%）牛奶样本呈阳性，而根据虎红试验（RBT），29份（19.7%）血清样本呈阳性；改良齐尔-尼尔森染色玻片显微镜检查检测出13.6%的病例，在布鲁氏菌培养基和胰蛋白胨大豆琼脂上布鲁氏菌属的回收率为7.5%。33份（22.4%）样本经PCR扩增IS711后呈阳性，这些样本随后被视为布鲁氏菌病阳性病例。RBT和PCR-IS711与MRT的差异具有高度显著性（P < 0.05）。与RBT、PCR、显微镜检查和培养相比，MRT检测出的牛布鲁氏菌病病例更多。与RBT相比，MRT被推荐为一种非侵入性检测方法，并且与PCR和培养相比成本更低。
