Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411 007, India.
ACS Appl Mater Interfaces. 2012 Feb;4(2):871-7. doi: 10.1021/am201543e. Epub 2012 Jan 17.
Silica nanostructures were phyto-fabricated on different surfaces by using pomegranate (Punica granatum) leaf extracts. On zinc films, nanowires were obtained. On other surfaces such as silica, alumina, zinc oxide, and glass, spherical aggregates, cubic assemblies, microflakes, and acicular structures, respectively, were observed. The nanowires developed on Zn surfaces were characterized by scanning electron microscope-energy dispersive spectrometer (SEM-EDS), X-ray diffraction (XRD), photoluminescence, and Fourier transform infrared (FTIR) spectroscopic analysis. XRD profiles displayed peaks at 2.4, 4.9, and 12.1° indicating the presence of silica nanostructures. When excited at 340 nm, the reaction mixtures displayed a characteristic blue luminescence at 404 nm. FTIR spectra showed the existence of Si-OH and Si-O-Si bonds. The nanowires were functionalized with amine groups and used for the covalent immobilization of Candida rugosa lipase. The immobilized enzyme displayed better pH and temperature stability and retained 80% activity after 20 cycles. This paper highlights a novel route for the phyto-mediated growth of silica nanowires on Zn surfaces, their characterization and effective use as a matrix for enzyme immobilization.
采用石榴（Punica granatum）叶提取物在不同表面上植物合成了硅纳米结构。在锌膜上获得了纳米线。在其他表面，如二氧化硅、氧化铝、氧化锌和玻璃上，分别观察到了球形聚集体、立方组装体、微薄片和针状结构。通过扫描电子显微镜-能量色散光谱仪（SEM-EDS）、X 射线衍射（XRD）、光致发光和傅里叶变换红外（FTIR）光谱分析对在 Zn 表面上开发的纳米线进行了表征。XRD 图谱在 2.4、4.9 和 12.1°处显示出表明存在硅纳米结构的峰。当在 340nm 处激发时，反应混合物在 404nm 处显示出特征蓝色发光。FTIR 光谱显示存在 Si-OH 和 Si-O-Si 键。纳米线被胺基官能化，并用于固定化 Candida rugosa 脂肪酶。固定化酶显示出更好的 pH 和温度稳定性，并且在 20 次循环后保留了 80%的活性。本文强调了一种在 Zn 表面上植物介导生长硅纳米线的新途径，以及它们的表征和作为酶固定化基质的有效利用。
