van den Ingh T S, Binkhorst G J, Kimman T G, Vreeswijk J, Pol J M, van Oirschot J T
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Zentralbl Veterinarmed B. 1990 Sep;37(7):532-8. doi: 10.1111/j.1439-0450.1990.tb01092.x.
A horse with neurological signs and severe meningoencephalitis caused by Aujeszky's disease is described. The diagnosis was established by immunohistochemistry, DNA-in situ hybridization and serological tests. Aujeszky's disease virus antigen and Aujeszky's disease viral DNA were detected in neurons of the cerebrum. In the serum of the horse antibodies against Aujeszky's disease virus were detected in a virus neutralization test, in a blocking ELISA which specifically detects antibodies against the glycoprotein I (Ig) of the virus, in an indirect double sandwich ELISA and with colloidal gold immunoelectron microscopy which detects antibodies directed against the envelope and nucleocapsid of the virus. Intranasal infection of two points with a high dose of Aujeszky's disease virus caused very wild and transient signs. Although the experimental infection induced virus neutralizing antibodies, it failed to induce gI specific antibodies.
本文描述了一匹患有由奥耶斯基氏病引起的神经症状和严重脑膜脑炎的马。通过免疫组织化学、DNA原位杂交和血清学检测确诊。在大脑神经元中检测到奥耶斯基氏病病毒抗原和奥耶斯基氏病病毒DNA。在该马的血清中，通过病毒中和试验、特异性检测针对该病毒糖蛋白I（Ig）抗体的阻断ELISA、间接双夹心ELISA以及检测针对该病毒包膜和核衣壳抗体的胶体金免疫电子显微镜检测到了抗奥耶斯基氏病病毒抗体。用高剂量奥耶斯基氏病病毒经鼻两点接种引起了非常剧烈且短暂的症状。尽管实验性感染诱导产生了病毒中和抗体，但未能诱导产生gI特异性抗体。
