Fu Shanji, Xiao Chen, Zhao Weiming, Yu Xiuping
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China.
Front Biosci (Elite Ed). 2012 Jan 1;4(5):1795-801. doi: 10.2741/e500.
Cancers are one of the main causes of human deaths globally. Great effort has been dedicated to the search for sensitive and specific markers of cancer. Polyamines including mainly putrescine (PUT), spermidine (SPD), and spermine (SP), are promising tumor markers since their excretion is frequently elevated in patients with various types of cancers. In the present study, we developed an efficient high performance liquid chromatographic (HPLC) method for the determination of polyamines in human serum using dansyl chloride for pre-column derivatization. All polyamines were separated within 10 min. The analytical method is simple, rapid, and highly reproducible. We applied of 11 cancers as objects of study, and made comparisons between polyamines and other 24 common tumor markers with six indexes: specificity, sensitivity, negative predictive value, positive predictive value, total effective, and the mean value. We drew the conclusion that polyamines are promising tumor markers, and they might be of great value in diagnosing cancers, predicting therapeutic success, or indicating the relapse of tumors.
癌症是全球人类死亡的主要原因之一。人们一直在努力寻找敏感且特异的癌症标志物。多胺主要包括腐胺（PUT）、亚精胺（SPD）和精胺（SP），是很有前景的肿瘤标志物，因为在各类癌症患者中它们的排泄量常常会升高。在本研究中，我们开发了一种高效的高效液相色谱（HPLC）方法，用于测定人血清中的多胺，该方法使用丹磺酰氯进行柱前衍生化。所有多胺在10分钟内即可分离。该分析方法简单、快速且重现性高。我们以11种癌症为研究对象，用特异性、敏感性、阴性预测值、阳性预测值、总有效率和平均值这六个指标，对多胺与其他24种常见肿瘤标志物进行了比较。我们得出结论，多胺是很有前景的肿瘤标志物，它们在癌症诊断、预测治疗效果或指示肿瘤复发方面可能具有重要价值。
