一种用于游离、结合和结合态多胺鉴定与定量的窄径高效液相色谱法。
A narrow-bore HPLC method for the identification and quantitation of free, conjugated, and bound polyamines.
作者信息
Kotzabasis K, Christakis-Hampsas M D, Roubelakis-Angelakis K A
机构信息
Department of Biology, University of Crete, Heraklion, Greece.
出版信息
Anal Biochem. 1993 Nov 1;214(2):484-9. doi: 10.1006/abio.1993.1526.
A modified sensitive high-pressure liquid chromatography (HPLC) method, using a narrow-bore column (2.1 x 200 mm, C-18, 5-microns particle size) with a methanol:water gradient (55-84%, v/v), is described for direct analysis of benzoylated free (S), soluble-conjugated (SH), and insoluble-bound (PH) polyamines in plant tissues. Regression curves for each fraction of polyamines allow a precise quantitative determination of putrescine, cadaverine, spermidine, spermine, and agmatine in all fractions (S, SH, PH) of a crude extract. A comparison of the results to the corresponding values for dansylated polyamines separated by thin-layer chromatography and quantitated by fluorometry is also presented. S, SH, and PH polyamines as benzoylated derivatives from leaves of Vitis vinifera L. cv Dogridge were characterized and quantitatively determined with this improved sensitive HPLC method.
本文描述了一种改进的灵敏高效液相色谱（HPLC）方法，该方法使用窄径柱（2.1×200 mm，C-18，粒径5微米），以甲醇：水梯度（55 - 84%，v/v），用于直接分析植物组织中苯甲酰化的游离（S）、可溶性共轭（SH）和不溶性结合（PH）多胺。多胺各组分的回归曲线可对粗提物所有组分（S、SH、PH）中的腐胺、尸胺、亚精胺、精胺和胍丁胺进行精确的定量测定。还给出了将结果与通过薄层色谱分离并用荧光法定量的丹磺酰化多胺的相应值进行比较的情况。用这种改进的灵敏HPLC方法对葡萄品种多瑞吉（Vitis vinifera L. cv Dogridge）叶片中的S、SH和PH多胺作为苯甲酰化衍生物进行了表征和定量测定。
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