Department of Microbial Biotechnology, Bharathiar University, Coimbatore 641 046, India.
J Microbiol. 2011 Dec;49(6):920-6. doi: 10.1007/s12275-011-1260-5. Epub 2011 Dec 28.
A new actinomycete strain, isolated from humus soils in the Western Ghats, was found to be an efficient pigment producer. The strain, designated AAA5, was identified as a putative Streptomyces aurantiacus strain based on cultural properties, morphology, carbon source utilization, and analysis of the 16S rRNA gene. The strain produced a reddish-brown pigmented compound during the secondary metabolites phase. A yellow compound was derived from the extracted pigment and was identified as the quinone-related antibiotic resistomycin based on ultraviolet-visible spectrophotometry, fourier transform infrared spectroscopy, liquid chromatography and mass spectroscopy, and nuclear magnetic resonance analyses. The AAA5 strain was found to produce large quantities of resistomycin (52.5 mg/L). It showed potent cytotoxic activity against cell lines viz. HepG2 (hepatic carcinoma) and HeLa (cervical carcinoma) in vitro, with growth inhibition (GI(50)) of 0.006 and 0.005 μg/ml, respectively. The strain also exhibited broad antimicrobial activities against both Gram-positive and Gram-negative bacteria. Therefore, AAA5 may have great potential as an industrial resistomycin-producing strain.
从西高止山脉腐殖土中分离到的一株放线菌被发现是一种高效的色素产生菌。该菌株被命名为 AAA5，根据培养特性、形态学、碳源利用和 16S rRNA 基因分析，被鉴定为潜在的橙色链霉菌菌株。该菌株在次级代谢物阶段产生一种红棕色色素化合物。从提取的色素中衍生出一种黄色化合物，并根据紫外可见分光光度法、傅里叶变换红外光谱、液相色谱和质谱以及核磁共振分析，将其鉴定为醌类抗生素雷多霉素。AAA5 菌株产生大量的雷多霉素（52.5mg/L）。它对 HepG2（肝癌）和 HeLa（宫颈癌）细胞系表现出很强的细胞毒性活性，体外生长抑制（GI(50)）分别为 0.006 和 0.005μg/ml。该菌株还对革兰氏阳性菌和革兰氏阴性菌均表现出广泛的抗菌活性。因此，AAA5 可能具有作为工业生产雷多霉素菌株的巨大潜力。
