Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
J Microbiol. 2011 Dec;49(6):1058-62. doi: 10.1007/s12275-011-1088-z. Epub 2011 Dec 28.
Group C rotaviruses are an important cause of acute gastroenteritis in humans and animals. Fecal samples were collected from a porcine herd in July, 2009. Group C rotavirus RNA was detected using RT-PCR for the VP6 gene. The identified strain was further characterized by sequencing and phylogenetic analysis of the partial VP4, and complete VP6 and VP7 gene sequences. The partial VP4 and complete VP6 gene sequences of the CUK-5 strain were most closely related to those of the CUK-6 strain of group C rotaviruses. Phylogenetic analysis of the VP7 gene of the 2 strains (CUK-5 and CUK-6) and reference strains of group G rotavirus by the neighbor-joining method also confirmed that CUK-5 and CUK-6 belonged to type G5 and G1 strains, respectively. This study provides useful data for the prediction of newly appearing variants of porcine group C rotaviruses in neighboring countries through comparisons with GCRVs and fundamental research for vaccine development.
C 组轮状病毒是人畜急性肠胃炎的重要病因。2009 年 7 月，采集了一个猪群的粪便样本。采用 RT-PCR 针对 VP6 基因检测 C 组轮状病毒 RNA。通过对部分 VP4、完整 VP6 和 VP7 基因序列的测序和系统发生分析，对鉴定出的毒株进行了进一步的特征描述。CUK-5 株的部分 VP4 和完整 VP6 基因序列与 C 组轮状病毒 CUK-6 株最为接近。采用邻接法对 2 株（CUK-5 和 CUK-6）和参考的 G 组轮状病毒的 VP7 基因进行的系统发生分析也证实，CUK-5 和 CUK-6 分别属于 G5 和 G1 型。通过与 GCRVs 的比较，本研究为预测邻国新生的猪 C 组轮状病毒变异株提供了有用的数据，也为疫苗开发提供了基础性研究。
