Ma Jianqun, Holden Kyle, Zhu Jinhong, Pan Haiying, Li Yong
The Laboratory of Molecular Pathology, Stem Cell Research Center, Children's Hospital of Pittsburgh, PA 15219, USA.
J Biomed Biotechnol. 2011;2011:812135. doi: 10.1155/2011/812135. Epub 2011 Dec 12.
Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graft in vitro. The 3D graft created in vitro was then utilized to investigate muscle tissue repair in vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graft in vitro that can be employed for defect muscle tissue repair in vivo.
三维（3D）工程化组织构建体是一种用于组织修复和再生的新颖且有前景的方法。与以往的方法（如整形外科手术，只能恢复外形，而软组织功能往往受损）不同，3D组织构建体有能力恢复受损软组织的形态和功能。在本研究中，我们将小鼠成肌细胞（C2C12）接种到胶原复合支架中，并在转瓶细胞培养系统中培养该支架，以便在体外创建3D组织移植物。然后利用体外创建的3D移植物来研究体内肌肉组织修复情况。将3D肌肉移植物植入小鼠骨骼肌中创建的缺损部位。我们检测到支架随时间缓慢降解，并且肌肉愈合得到改善，这表现为有神经支配和血管化的再生肌纤维数量增加。我们的结果表明，接种有成肌细胞的胶原复合支架能够在体外创建可用于体内缺损肌肉组织修复的3D肌肉移植物。
