Teixeira Vera, Arede Natacha, Gardner Rui, Rodríguez-León Joaquín, Tavares Ana T
Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal.
BMC Dev Biol. 2011 Dec 28;11:76. doi: 10.1186/1471-213X-11-76.
Hemangioblasts are known as the common precursors for primitive hematopoietic and endothelial lineages. Their existence has been supported mainly by the observation that both cell types develop in close proximity and by in vitro differentiation and genetic studies. However, more compelling evidence will arise from tracking their cell fates using a lineage-specific marker.
We report the identification of a hemangioblast-specific enhancer (Hb) located in the cis-regulatory region of chick Cerberus gene (cCer) that is able to direct the expression of enhanced green fluorescent protein (eGFP) to the precursors of yolk sac blood and endothelial cells in electroporated chick embryos. Moreover, we present the Hb-eGFP reporter as a powerful live imaging tool for visualizing hemangioblast cell fate and blood island morphogenesis.
We hereby introduce the Hb enhancer as a valuable resource for genetically targeting the hemangioblast population as well as for studying the dynamics of vascular and blood cell development.
成血管细胞被认为是原始造血和内皮谱系的共同前体。它们的存在主要通过观察到这两种细胞类型在相邻位置发育以及通过体外分化和遗传学研究得到支持。然而，使用谱系特异性标记追踪它们的细胞命运将产生更有说服力的证据。
我们报告了在鸡Cerberus基因（cCer）的顺式调控区域中鉴定出一种成血管细胞特异性增强子（Hb），它能够将增强型绿色荧光蛋白（eGFP）的表达导向电穿孔鸡胚中卵黄囊血液和内皮细胞的前体。此外，我们展示了Hb-eGFP报告基因作为一种强大的实时成像工具，用于可视化成血管细胞的命运和血岛形态发生。
我们在此引入Hb增强子，作为一种有价值的资源，用于对成血管细胞群体进行基因靶向以及研究血管和血细胞发育的动态过程。
