Resource Management and Environmental Studies, University of British Columbia, Vancouver, Canada.
Int J Hyg Environ Health. 2012 Apr;215(3):270-8. doi: 10.1016/j.ijheh.2011.12.004. Epub 2011 Dec 26.
Despite its relevance to public health, presence and concentrations of Campylobacter spp. in biofilms in natural aquatic environments has not been investigated. This study examined the occurrence of Campylobacter spp. in biofilms on a variety of surfaces (river rock, slate rock, wood, Lexan™, sandpaper, and sediment) and in water from December 2005 to December 2006 to find a substratum that facilitated campylobacters detection in natural aquatic environments. Samples were collected at four sites in an agricultural watershed (Elk Creek, British Columbia). Campylobacter spp. presence was determined using culturing methods. Correlations between chemical, physical and microbiological water quality parameters and Campylobacter spp. distribution on different surface types were also investigated. Campylobacter spp. had a prevalence of 13% in the wet season, but was not recovered in the dry season. Its prevalence was highest in sediment (27%), followed by slate rock (22%), Lexan and wood (13%), river rock (9%) and water (8%), respectively. No Campylobacter spp. was found in sandpaper biofilms. Several other criteria were used to assess substrata effectiveness, such as correlation amongst Campylobacter spp., indicator bacteria and water quality parameters, cost and availability of substratum, potential for standardizing substratum, ease of biofilm removal and probability of substratum loss in situ. Results show that sediment, slate rock or wood could be used as substrata for Campylobacter spp. monitoring. The study also highlights the potential use of nitrates and enterococci as faecal contamination indicators to protect public health.
尽管弯曲菌属在公共卫生方面具有相关性，但在自然水生环境中的生物膜中存在和浓度尚未得到研究。本研究调查了弯曲菌属在各种表面（河石、板岩、木材、Lexan™、砂纸和沉积物）上的生物膜以及 2005 年 12 月至 2006 年 12 月的水中的出现情况，以寻找一种有利于在自然水生环境中检测弯曲菌的基质。样品取自农业流域（不列颠哥伦比亚省的 Elk Creek）的四个地点采集。使用培养方法确定弯曲菌属的存在。还研究了水化学、物理和微生物学水质参数与不同表面类型上弯曲菌属分布之间的相关性。弯曲菌属在湿季的流行率为 13%，但在干季未被回收。其流行率在沉积物中最高（27%），其次是板岩（22%）、Lexan 和木材（13%）、河石（9%）和水（8%）。在砂纸生物膜中未发现弯曲菌属。还使用了其他几个标准来评估基质的有效性，例如弯曲菌属、指示菌和水质参数之间的相关性、基质的成本和可用性、基质标准化的潜力、生物膜去除的容易程度以及基质在原位丢失的可能性。结果表明，沉积物、板岩或木材可用作弯曲菌属监测的基质。该研究还强调了硝酸盐和肠球菌作为粪便污染指标的潜在用途，以保护公众健康。
