Department of Otolaryngology Head and Neck Surgery Assaf Harofeh Medical Center, Zerifin, Israel.
J Occup Med Toxicol. 2011 Dec 29;6:38. doi: 10.1186/1745-6673-6-38.
Exposure to continuous and impulse noise can induce a hearing loss. Leupeptin is an inhibitor of the calpains, a family of calcium-activated proteases which promote cell death. The objective of this study is to assess whether Leupeptin could reduce the hearing loss resulting from rifle impulse noise.
A polyethelene tube was implanted into middle ear cavities of eight fat sand rats (16 ears). Following determination of auditory nerve brainstem evoked response (ABR) threshold in each ear, the animals were exposed to the noise of 10 M16 rifle shots. Immediately after the exposure, saline was then applied to one (control) ear and non-toxic concentrations of leupeptin determined in the first phase of the study were applied to the other ear, for four consecutive days.
Eight days after the exposure, the threshold shift (ABR) in the control ears was significantly greater (44 dB) than in the leupeptin ears (27 dB).
Leupeptin applied to the middle ear cavity can reduce the hearing loss resulting from exposure to impulse noise.
接触连续和脉冲噪声会导致听力损失。亮抑酶肽是钙蛋白酶的抑制剂,钙蛋白酶是一种钙激活的蛋白酶家族,可促进细胞死亡。本研究的目的是评估亮抑酶肽是否可以减轻来福枪脉冲噪声引起的听力损失。
将聚乙稀管植入 8 只肥沙鼠的中耳腔(16 耳)。在确定每只耳朵的听神经脑干诱发电位(ABR)阈值后,将动物暴露于 10 支 M16 来福枪声中。暴露后,立即将生理盐水应用于一只(对照)耳朵,而在第一阶段研究中确定的无毒浓度的亮抑酶肽则连续四天应用于另一只耳朵。
暴露后 8 天,对照耳的阈值移位(ABR)明显大于亮抑酶肽耳(44 dB)(27 dB)。
亮抑酶肽应用于中耳腔可减轻因暴露于脉冲噪声引起的听力损失。