Institute for Biochemical Engineering, University of Stuttgart, Allmandring 31, Stuttgart, Germany.
J Biotechnol. 2012 Jul 31;160(1-2):3-9. doi: 10.1016/j.jbiotec.2011.12.010. Epub 2011 Dec 21.
Industrial biotechnological production is developing rapidly worldwide. Consequently, more and novel bioprocesses need to perform optimally not only in small lab- but also in large production scales. This article shortly reviews typical impacts found when cells are exposed to micro environmental heterogeneities typically occurring in poorly mixed large scale production reactors. The current state-of-the-art of tool development is presented for analyzing these phenomena. Finally, still open questions are formulated and needs for future research are outlined to further support the expansion of biotech industries by successful research results.
工业生物技术生产在全球范围内迅速发展。因此，不仅需要在小实验室规模下，而且需要在大规模生产规模下使更多新型生物工艺实现最佳性能。本文简要回顾了细胞暴露于在混合不佳的大规模生产反应器中典型发生的微环境非均一性时所发现的典型影响。为分析这些现象呈现了当前工具开发的最新技术。最后，提出了尚未解决的问题并概述了未来研究的需求，以通过成功的研究结果进一步支持生物技术产业的扩展。
