Department of Medical Genetics, Faculty of Medicine, Uludag University, 16059, Gorukle, Bursa, Turkey.
Mol Biol Rep. 2012 May;39(5):6187-91. doi: 10.1007/s11033-011-1436-4. Epub 2011 Dec 30.
A common observation after in vitro matured oocyte is that they yield poorer embryo quality compared to their in vivo counterparts. This study was designed to assess chromosomal status with metaphase comparative genomic hybridization after in vitro maturation (IVM) in unstimulated cycles and compare the results with those obtained after in vivo maturation. Patients without any obstetrical or gynecological pathology were admitted into the study. IVM oocytes were collected 36 h post hCG and matured in vitro at 37°C in 5% O(2), 6% CO(2), and 89% air for 36 h. All matured (metaphase II) oocytes were subject to polar body 1 (PB-1) biopsy and vitrified individually. PB-1 samples were transferred into 0.25 cc PCR tubes containing 2.5 μl of PBS. PB-1 samples from 12 IVM patients were studied. Twenty-six out of 63 PB-1 samples (41%) were determined as euploid and 37 samples (59%) were aneuploid, whereas these values were 42% euploid and 58% aneuploid in the control group (in vivo matured oocytes). No statistical differences were found between the IVM and the control groups for euploid-aneuploid samples (P = 0.900). More aneuploidy was observed on chromosomes 11, 13, 15, 21, and 22 after IVM. Results show a non-significant rate of abnormal PB-1 formation after IVM compared to in vivo maturation. More aneuploidy was observed in chromosomes 11, 13, 15, 21, and 22 in the IVM group.
体外成熟卵母细胞的一个常见观察结果是，与体内成熟卵母细胞相比，它们产生的胚胎质量较差。本研究旨在评估体外成熟（IVM）后未刺激周期中的中期比较基因组杂交的染色体状态，并将结果与体内成熟后获得的结果进行比较。没有任何产科或妇科病史的患者被纳入研究。IVM 卵母细胞在 hCG 后 36 小时采集，并在 37°C 下在 5% O(2)、6% CO(2)和 89%空气中体外成熟 36 小时。所有成熟（中期 II）卵母细胞均进行极体 1（PB-1）活检并单独冷冻。PB-1 样本转移到含有 2.5μl PBS 的 0.25cc PCR 管中。对 12 名 IVM 患者的 PB-1 样本进行了研究。26 个 PB-1 样本（41%）被确定为整倍体，37 个样本（59%）为非整倍体，而对照组（体内成熟卵母细胞）中这些值分别为 42%整倍体和 58%非整倍体。IVM 组和对照组在整倍体-非整倍体样本之间未发现统计学差异（P=0.900）。IVM 后观察到染色体 11、13、15、21 和 22 的非整倍体增加。结果表明，与体内成熟相比，IVM 后 PB-1 形成的异常率没有显著增加。在 IVM 组中观察到染色体 11、13、15、21 和 22 的非整倍体增加更多。
