School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.
Biosens Bioelectron. 2012 Feb 15;32(1):293-6. doi: 10.1016/j.bios.2011.12.006. Epub 2011 Dec 13.
Selective detection of cysteine in serum samples was achieved on a graphene nanoribbon (GNR) and Nafion nanocomposite modified electrode with high precision. The superior conductivity and abundant amount of active chemical oxygen groups on the edge of GNR led to extremely highly electrocatalytic activity of GNR towards the electrochemical oxidation of cysteine at +0.025 V. The electrocatalytic behavior was further used for sensitive detection of cysteine by differential pulse voltammetry. Under optimized conditions, the calibration curve was linear in the range from 25 nM to 500 μM. The electrochemical sensor showed strong antifouling ability, good stability and selectivity. It could effectively exclude the interferences from other kinds of biothiols and the biological relevant species, thus had great perspective for in vivo analysis of biological samples.
基于高度精准的石墨烯纳米带（GNR）和 Nafion 纳米复合材料修饰电极，实现了对血清样本中半胱氨酸的选择性检测。GNR 具有优异的导电性和丰富的边缘活性化学氧基团，使其对电化学氧化半胱氨酸具有极高的电催化活性，在+0.025 V 处进行检测。该电催化行为进一步通过差分脉冲伏安法用于对半胱氨酸的灵敏检测。在优化条件下，校准曲线在 25 nM 至 500 μM 的范围内呈线性关系。电化学传感器表现出很强的抗污能力、良好的稳定性和选择性。它可以有效地排除其他种类生物硫醇和生物相关物质的干扰，因此在生物样品的体内分析方面具有广阔的应用前景。
