Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX 77555-0435, USA.
Immunobiology. 2012 Jul;217(7):711-8. doi: 10.1016/j.imbio.2011.12.002. Epub 2011 Dec 8.
The effect of IL-10 antisense oligodeoxynucleotides (ODN) on the susceptibility of burned mice to intradermal (i.d.) infection of methicillin-resistant Staphylococcus aureus (MRSA) was studied. Abscesses formed and sepsis did not develop in normal mice infected i.d. with 10(8)CFU/mouse of MRSA. Similarly, sepsis caused by MRSA i.d. infection did not develop and abscesses formed in burned mice treated with IL-10 antisense ODN. However, all of the burned mice treated with scrambled ODN (control group) died by infectious complications stemming from MRSA i.d. infection, and an MRSA-abscess did not form in these mice. Macrophages (Mϕ) isolated from the infection site tissue of burned mice that were treated with IL-10 antisense ODN were identified as M1Mϕ, while Mϕ isolated from burned mice that were treated with scrambled ODN were shown to be M2Mϕ. MRSA-abscesses formed in burned mice inoculated with M1Mϕ, and these mice resisted a lethal dose of MRSA i.d. infection. However, an abscess did not form, and sepsis caused by MRSA i.d. infection developed in burned mice that were inoculated with M2Mϕ. These results indicate that severely burned mice treated with IL-10 antisense ODN are resistant against i.d. infection with MRSA. M1Mϕ appeared in the infection site tissues of severely burned mice that were treated with IL-10 antisense ODN may play a role on the abscess formation and inhibiting sepsis caused by MRSA i.d. infection.
研究了白细胞介素-10 反义寡脱氧核苷酸（ODN）对烧伤小鼠对耐甲氧西林金黄色葡萄球菌（MRSA）皮内（i.d.）感染易感性的影响。在正常小鼠中，用 10（8）CFU/只的 MRSA 感染 i.d.，会形成脓肿且不会发生败血症。同样，用 IL-10 反义 ODN 处理的烧伤小鼠感染 MRSA 后，也不会发生败血症，且脓肿形成。然而，所有用乱序 ODN（对照组）处理的烧伤小鼠都因 MRSA i.d.感染的感染并发症而死亡，这些小鼠中未形成 MRSA 脓肿。从用 IL-10 反义 ODN 处理的烧伤小鼠感染部位组织中分离出的巨噬细胞（Mϕ）被鉴定为 M1Mϕ，而从用乱序 ODN 处理的烧伤小鼠中分离出的 Mϕ被鉴定为 M2Mϕ。在接种 M1Mϕ 的烧伤小鼠中形成了 MRSA 脓肿，这些小鼠能够抵抗致死剂量的 MRSA i.d.感染。然而，在接种 M2Mϕ 的烧伤小鼠中，未形成脓肿，但发生了由 MRSA i.d.感染引起的败血症。这些结果表明，用 IL-10 反义 ODN 处理的严重烧伤小鼠对耐甲氧西林金黄色葡萄球菌的 i.d.感染具有抗性。在接受 IL-10 反义 ODN 治疗的严重烧伤小鼠的感染部位组织中出现的 M1Mϕ 可能在脓肿形成和抑制由 MRSA i.d.感染引起的败血症方面发挥作用。
