Dipartimento di Biologia e Chimica Agro-forestale e Ambientale, University of Bari, Via Amendola 165/A, 70126 Bari, Italy.
Sci Total Environ. 2012 Feb 1;416:501-6. doi: 10.1016/j.scitotenv.2011.11.033. Epub 2011 Dec 29.
Phytoremediation of waters by aquatic organisms such as algae has been recently explored for the removal of organic pollutants possessing endocrine disrupting capacity. Monoraphidium braunii, a green alga known for rapid growth and good tolerance to different natural organic matter (NOM) qualities, was tested in this study for the ability to tolerate and remove the endocrine disruptor bisphenol A at concentrations of 2, 4 and 10mgL(-1), either in NOM-free or NOM-containing media. NOM at concentrations of 2, 5 and 20mgL(-1) of DOC, was added because it may interfere with xenobiotics and modify their effects, modulate algal growth performances or produce a trade-off of both effects. After 2 and 4 days of algal growth, the cell number and size, the maximum quantum yield of photosystem II in the dark or light adapted state, and the chlorophyll a content were recorded in order to evaluate the algal response to bisphenol A. Moreover, the residual bisphenol A was measured in the algal cultures by chromatographic technique. Results indicated that after 2 and 4 days bisphenol A at the lower concentrations was not toxic for alga, whereas at the highest concentration it reduced algal growth and photosynthetic efficiency. The sole NOM and its combinations with bisphenol A at the lower concentrations increased the cell number and the chlorophyll a content of algae. After 4-day growth, good removal efficiency was exerted by M. braunii at concentrations of 2, 4 and 10mgL(-1) removing, respectively, 39%, 48% and 35% of the initial bisphenol A. Lower removal percentages were found after 2-day growth in the different treatments. NOM at any concentration scarcely influenced the bisphenol A removal. On the basis of data obtained, the use of M. braunii could be reasonably recommended for the phytoremediation of aquatic environments from bisphenol A.
水生生物（如藻类）对水体的植物修复最近被用于去除具有内分泌干扰能力的有机污染物。微拟球藻是一种生长迅速、对不同天然有机物（NOM）质量具有良好耐受性的绿藻，本研究测试了其在无 NOM 或含 NOM 介质中耐受和去除内分泌干扰物双酚 A 的能力，浓度分别为 2、4 和 10mgL(-1)。添加浓度为 2、5 和 20mgL(-1)DOC 的 NOM 是因为它可能会干扰外来化合物并改变它们的作用，调节藻类的生长性能或产生这两种作用的权衡。在藻类生长 2 和 4 天后，记录细胞数量和大小、暗适应或光适应状态下光系统 II 的最大量子产量以及叶绿素 a 的含量，以评估藻类对双酚 A 的反应。此外，通过色谱技术测量藻类培养物中的残留双酚 A。结果表明，在较低浓度下，双酚 A 在 2 和 4 天后对藻类没有毒性，而在最高浓度下，它会降低藻类的生长和光合作用效率。单独的 NOM 及其与较低浓度的双酚 A 的组合会增加藻类的细胞数量和叶绿素 a 的含量。经过 4 天的生长，M. braunii 在 2、4 和 10mgL(-1)浓度下对双酚 A 的去除效率分别为 39%、48%和 35%，而在不同处理下，2 天后的去除率较低。在任何浓度下的 NOM 都很少影响双酚 A 的去除。根据获得的数据，M. braunii 可合理推荐用于从水生环境中去除双酚 A。
