Department of Chemical Engineering and Environmental Technology, University of Valladolid, Doctor Mergelina s/n, 47011 Valladolid, Spain.
Bioresour Technol. 2012 Mar;107:229-34. doi: 10.1016/j.biortech.2011.12.010. Epub 2011 Dec 13.
The chemical pretreatment with ozone of rye and wheat straws was carried out in a fixed bed reactor. The effect of ozone pretreatment time on lignin removal was determined. Glucose and xylose concentrations in the hydrolysates were also measured after the subsequent enzymatic hydrolysis step. Acid insoluble lignin reacts with ozone within the first 90 min reaction. Insoluble lignin reduction was about 50%. The higher hydrolysis yields were obtained after 120 min ozonation: the glucose yield ranged from 40% to 50% for rye straw and from 34% to 39% for wheat straw, whereas xylose yields were about 30%, independently of the cereal straw. The glucose yields corresponding to the untreated raw straws were considerably lower, about 10%. Longer ozonation time sharply reduced the production of monosaccharides, probably due to the formation of side products. The kinetic model, with the reaction parameters estimated, predicted reasonably well the experimental data.
采用固定床反应器对黑麦和小麦秸秆进行臭氧化学预处理，考察了臭氧预处理时间对木质素去除效果的影响。在后续的酶水解步骤后，还测量了水解产物中葡萄糖和木糖的浓度。酸不溶木质素在最初的 90 分钟反应中与臭氧反应。不溶性木质素的减少约为 50%。经过 120 分钟的臭氧氧化后，获得了更高的水解产率：黑麦秸秆的葡萄糖产率范围为 40%至 50%，小麦秸秆的葡萄糖产率范围为 34%至 39%，而木糖产率约为 30%，与谷物秸秆无关。未经处理的原秸秆的葡萄糖产率要低得多，约为 10%。较长的臭氧处理时间会急剧降低单糖的产量，这可能是由于副产物的形成。用估算出的反应参数建立的动力学模型能够很好地预测实验数据。
