Department of Chemistry and Institute of BioPhysico Sensor Technology, Pusan National University, Busan 609-735, South Korea.
Biosens Bioelectron. 2011 Jul 15;26(11):4442-9. doi: 10.1016/j.bios.2011.04.060. Epub 2011 May 6.
A highly sensitive and selective sensor for daunomycin was developed using phosphatidylserine (PS) and aptamer as bioreceptors. The PS and aptamer were co-immobilized onto gold nanoparticles modified/functionalized [2,2':5',2″-terthiophene-3'-(p-benzoic acid)] (polyTTBA) conducting polymer. Direct electrochemistry of daunomycin was used to fabricate a label free sensor that monitors current at -0.61 V. The formation of each layer was confirmed with XPS, SEM, and QCM. Response of the sensor was compared with and without PS in terms of sensitivity and selectivity. Interaction between the sensor probe and daunomycin was determined with DPV. The experimental parameters affecting sensor performance were optimized in terms of concentration of immobilized aptamer, PS:aptamer ratio, temperature, pH, and reaction times. The dynamic range for daunomycin analysis ranged between 0.1 and 60.0 nM with a detection limit of 52.3 ± 2.1 pM. Sensor was also examined for interference effect of other drugs. The present sensor exhibited long term stability and successfully detected daunomycin in a real human urine spiked with daunomycin.
采用磷脂酰丝氨酸（PS）和适体作为生物受体，研制了一种对柔红霉素具有高灵敏度和选择性的传感器。PS 和适体共同固定在修饰/功能化的 [2,2':5',2″-三联噻吩-3'-(对苯甲酸)]（聚 TTBA）导电聚合物修饰的金纳米粒子上。直接电化学法用于制备无标记传感器，以监测-0.61 V 的电流。通过 XPS、SEM 和 QCM 确认了每一层的形成。从灵敏度和选择性方面比较了有 PS 和无 PS 时传感器的响应。通过 DPV 确定了传感器探针与柔红霉素之间的相互作用。根据固定化适体的浓度、PS:适体比、温度、pH 值和反应时间等实验参数对传感器性能进行了优化。柔红霉素分析的动态范围在 0.1 和 60.0 nM 之间，检测限为 52.3 ± 2.1 pM。还研究了传感器对其他药物的干扰效应。该传感器具有长期稳定性，成功地检测到添加柔红霉素的真实人尿样中的柔红霉素。
