Morovati Hassan, Shirvani Edris, Noaman Vahid, Lotfi Mohsen, Kamalzadeh Morteza, Hatami Alireza, Bahreyari Masoume, Shahramyar Zahra, Morovati Mohammad H, Azimi Mahmoud, Sakhaei Davoud
Quality Control Management, Razi Vaccine and Serum Research Institute, PO Box 311975-148, Karaj, Iran.
Trop Anim Health Prod. 2012 Aug;44(6):1127-9. doi: 10.1007/s11250-011-0062-4. Epub 2011 Dec 31.
Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis (EBL) is an exogenous C-type oncovirus in the Retroviridae family. It causes significant economic losses associated with the costs of control and eradication programs due to carcass condemnation at slaughter and restrictions of export of cattle and semen to importing countries. The main objective of this research was to determine the seroprevalence of BLV infection in cattle herds in central region of Iran (Isfahan province) using a commercial enzyme-linked immunosorbent assay (ELISA) to detect serum antibodies against BLV. Samples of blood serum were collected from 403 female dairy cattle (Holstein-Friesian) from 21 livestock farms and 303 animals (81.9%) were BLV seropositive. A significant association was found between age as a potential risk factor and BVL seroprevalence with animals ≥ 4 years (86.6%) having a significantly (χ(2) = 35.6, p < 0.001) higher seroprevalence compared to those < 4 years (54.2%). We found no significant statistical association between seroprevalence and pregnancy, lactation status and farming systems as potential risk factors in this study (p > 0.1). It is concluded that BLV infection is a very common problem in the study area. Hence, control measures should be instituted to combat the disease and further studies are required to investigate the impact of this disease on dairy production in the country.
牛白血病病毒（BLV）是地方流行性牛白血病（EBL）的病原体，属于逆转录病毒科的一种外源性C型肿瘤病毒。由于屠宰时胴体被判不合格以及牛和精液出口到进口国受到限制，它导致了与控制和根除计划成本相关的重大经济损失。本研究的主要目的是使用商业酶联免疫吸附测定（ELISA）检测牛血清中抗BLV抗体，以确定伊朗中部地区（伊斯法罕省）牛群中BLV感染的血清阳性率。从21个畜牧场的403头雌性奶牛（荷斯坦-弗里生牛）采集血清样本，其中303头动物（81.9%）为BLV血清阳性。作为潜在风险因素的年龄与BVL血清阳性率之间存在显著关联，4岁及以上的动物（86.6%）的血清阳性率显著高于4岁以下的动物（54.2%）（χ(2)=35.6，p<0.001）。在本研究中，我们发现血清阳性率与妊娠、泌乳状态和养殖系统作为潜在风险因素之间没有显著的统计学关联（p>0.1）。得出的结论是，BLV感染在研究区域是一个非常普遍的问题。因此，应采取控制措施来对抗这种疾病，并且需要进一步研究来调查这种疾病对该国奶牛生产的影响。
