Univ Paris-Sud, Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619, Orsay, F-91405, France.
Biochimie. 2012 Apr;94(4):985-90. doi: 10.1016/j.biochi.2011.12.019. Epub 2011 Dec 26.
Lipids II found in some Gram-positive bacteria were prepared in radioactive form from l-lysine-containing UDP-MurNAc-pentapeptide. The specific lateral chains of Enterococcus faecalis, Enterococcus faecium and Staphylococcus aureus (di-L-alanine, D-isoasparagine, and pentaglycine, respectively) were introduced by chemical peptide synthesis using the Fmoc chemistry. The branched nucleotides obtained were converted into the corresponding lipids II by enzymatic synthesis using the MraY and MurG enzymes. All of the lipids were hydrolysed by Escherichia coli colicin M at approximately the same rate as the meso-diaminopimelate-containing lipid II found in Gram-negative bacteria, thereby opening the way to the use of this enzyme as a broad spectrum antibacterial agent.
一些革兰氏阳性菌中的脂质 II 是从含有 l-赖氨酸的 UDP-MurNAc-五肽中以放射性形式制备的。通过使用 Fmoc 化学的化学肽合成，引入了肠球菌（粪肠球菌和屎肠球菌）和金黄色葡萄球菌（分别为二-L-丙氨酸、D-异天冬酰胺和五甘氨酸）的特定侧链。使用 MraY 和 MurG 酶的酶促合成将得到的支化核苷酸转化为相应的脂质 II。所有的脂质都被大肠杆菌 colicin M 以大约与革兰氏阴性细菌中含有 meso-二氨基庚二酸的脂质 II 相同的速率水解，从而为该酶作为广谱抗菌剂的使用开辟了道路。
