CSIRO Plant Industry, Wembley, Perth, WA 6913, Australia.
Mol Plant Pathol. 2012 Aug;13(6):593-603. doi: 10.1111/j.1364-3703.2011.00767.x. Epub 2012 Jan 3.
Gene expression changes and metabolite abundances were measured during the interaction of Medicago truncatula with the fungal necrotrophic pathogen Phoma medicaginis in leaf tissue of susceptible and resistant accessions. Over 330 genes were differentially expressed in plants infected with P. medicaginis relative to mock-inoculated plants at 12 h post-inoculation. Of these, 191 were induced in either the resistant or the susceptible accession, with 143 genes repressed. Expression changes were observed in genes involved in the oxidative burst, cell wall strengthening and lipid metabolism, as well as several transcription factors. Genes related to salicylic acid, jasmonate and ethylene responses were up-regulated, as well as genes leading to the production of jasmonic acid. Significant induction of genes in the phenylpropanoid pathway leading to lignin and isoflavonoid biosynthesis occurred. High-pressure liquid chromatography with UV detection (HPLC-UV) identified several phenolic compounds induced by P. medicaginis, as well as constitutively higher levels of phenolic compounds, in the resistant M. truncatula accession. Differentially regulated genes induced in both the resistant and susceptible accessions, but with different kinetics, and constitutively more highly expressed and induced phenolic compounds provide candidates for functional analysis. Taken together, these results highlight the importance of the octadecanoid and phenylpropanoid pathways in defence against this necrotrophic pathogen.
在豌豆与真菌坏死病原体 Phoma medicaginis 互作过程中，测量了叶片组织中基因表达变化和代谢物丰度。与假接种植物相比，接种 P. medicaginis 12 小时后，超过 330 个基因在感病和抗病品种中差异表达。其中，191 个基因在感病或抗病品种中被诱导，143 个基因被抑制。观察到与氧化爆发、细胞壁强化和脂质代谢以及几个转录因子相关的基因表达变化。与水杨酸、茉莉酸和乙烯反应相关的基因上调，以及导致茉莉酸产生的基因上调。木质素和异黄酮生物合成的苯丙烷途径中的基因显著诱导。用紫外线检测的高压液相色谱法 (HPLC-UV) 鉴定了由 P. medicaginis 诱导的几种酚类化合物，以及在抗病 M. truncatula 品种中酚类化合物的组成型更高水平。在感病和抗病品种中诱导的差异调节基因，但动力学不同，以及组成型更高表达和诱导的酚类化合物为功能分析提供了候选物。综上所述，这些结果强调了十八碳烯酸和苯丙烷途径在防御这种坏死病原体中的重要性。
