Department of Orthopedic Surgery, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan.
J Orthop Res. 2012 Jul;30(7):1103-11. doi: 10.1002/jor.22044. Epub 2011 Dec 27.
Calcium phosphate cement (CPC) is reported to have excellent biocompatibility and osteoconductivity. However, its biodegradability must be improved to promote bone regeneration. We have mixed gelatin powder with CPC to create a composite containing macropores with interconnectivity. Sixty rabbits were grouped as follows: 85 wt% CPC to 15 wt% gelatin powder (C15), 90 wt% CPC to 10 wt% gelatin powder (C10), 100 wt% CPC (C0) as control group and Sham group. Trabecular bone defects of distal femurs were made and implanted with the composites. The femurs were harvested for histomorphometry at 4, 12, 24 weeks after implantation, and mechanical testing at 3 days, 1, 4, 12, 24 weeks. Compared with C0, X-ray and micro-CT results of the composites revealed a progressive increase in the amount of CPC-gelatin powder composite which was replaced by trabeculae. New bone area increased from 3.8 to 18% in C10, and 4.2 to 22% in C15, residual composite area decreased from 65 to 31% in C10, and 70 to 20% in C15. The compressive strength of C15 was 9.2 MPa, which was inferior to 14.6 MPa (normal cancellous bone), but was 27.4 MPa in C10 at 1 week. Further improvement of this composite may make a suitable scaffold for bone regeneration.
磷酸钙骨水泥（CPC）具有良好的生物相容性和骨诱导性。然而，其生物降解性有待提高，以促进骨再生。我们将明胶粉末与 CPC 混合，制成具有互连通孔的复合材料。将 60 只兔子分为以下几组：85wt%的 CPC 和 15wt%的明胶粉末（C15）、90wt%的 CPC 和 10wt%的明胶粉末（C10）、100wt%的 CPC（C0）作为对照组和假手术组。在兔股骨远端骨小梁缺损处植入复合材料。植入后 4、12、24 周时取股骨进行组织形态计量学检测，植入后 3 天、1、4、12、24 周时进行力学测试。与 C0 相比，复合材料的 X 射线和微 CT 结果显示，CPC-明胶粉末复合材料的量逐渐增加，逐渐被骨小梁取代。新骨面积从 C10 的 3.8%增加到 18%，从 C15 的 4.2%增加到 22%，残余复合材料面积从 C10 的 65%减少到 31%，从 C15 的 70%减少到 20%。C15 的抗压强度为 9.2MPa，低于正常松质骨的 14.6MPa，但在 C10 中为 1 周时的 27.4MPa。进一步改进这种复合材料可能会使其成为一种合适的骨再生支架。
