Institute of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.
PLoS One. 2013 Apr 25;8(4):e62570. doi: 10.1371/journal.pone.0062570. Print 2013.
Calcium phosphate cement (CPC) can be molded or injected to form a scaffold in situ, which intimately conforms to complex bone defects. Bioactive glass (BG) is known for its unique ability to bond to living bone and promote bone growth. However, it was not until recently that literature was available regarding CPC-BG applied as an injectable graft. In this paper, we reported a novel injectable CPC-BG composite with improved properties caused by the incorporation of BG into CPC.
The novel injectable bioactive cement was evaluated to determine its composition, microstructure, setting time, injectability, compressive strength and behavior in a simulated body fluid (SBF). The in vitro cellular responses of osteoblasts and in vivo tissue responses after the implantation of CPC-BG in femoral condyle defects of rabbits were also investigated.
CPC-BG possessed a retarded setting time and markedly better injectability and mechanical properties than CPC. Moreover, a new Ca-deficient apatite layer was deposited on the composite surface after immersing immersion in SBF for 7 days. CPC-BG samples showed significantly improved degradability and bioactivity compared to CPC in simulated body fluid (SBF). In addition, the degrees of cell attachment, proliferation and differentiation on CPC-BG were higher than those on CPC. Macroscopic evaluation, histological evaluation, and micro-computed tomography (micro-CT) analysis showed that CPC-BG enhanced the efficiency of new bone formation in comparison with CPC.
A novel CPC-BG composite has been synthesized with improved properties exhibiting promising prospects for bone regeneration.
磷酸钙水泥 (CPC) 可成型或注射以原位形成支架，与复杂的骨缺损紧密贴合。生物活性玻璃 (BG) 以其独特的与活骨结合并促进骨生长的能力而闻名。然而，直到最近，才出现了关于将 CPC-BG 用作可注射移植物的文献。在本文中，我们报告了一种新型可注射 CPC-BG 复合材料，由于将 BG 掺入 CPC 中，其性能得到了改善。
评估了新型可注射生物活性水泥，以确定其组成、微观结构、凝固时间、可注射性、抗压强度和在模拟体液 (SBF) 中的行为。还研究了成骨细胞的体外细胞反应和 CPC-BG 植入兔股骨髁缺损后的体内组织反应。
CPC-BG 具有延迟凝固时间、明显更好的可注射性和机械性能优于 CPC。此外，在 SBF 浸泡 7 天后，复合材料表面沉积了一层新的缺钙磷灰石层。与 CPC 相比，CPC-BG 在模拟体液 (SBF) 中表现出显著改善的降解性和生物活性。此外，CPC-BG 上的细胞附着、增殖和分化程度高于 CPC。宏观评估、组织学评估和微计算机断层扫描 (micro-CT) 分析表明，与 CPC 相比，CPC-BG 增强了新骨形成的效率。
已经合成了具有改善性能的新型 CPC-BG 复合材料，为骨再生展现了广阔的前景。
