Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH-3010 Berne, Switzerland.
J Biomed Mater Res A. 2012 Mar;100(3):703-11. doi: 10.1002/jbm.a.34004. Epub 2011 Dec 30.
Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine-glycine-aspartic acid (RGD) and lysine-arginine-serine-arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid-etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly-L-lysine-graft-poly(ethylene glycol) (PLL-g-PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm⁻² KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm⁻² RGD (KRSR/RGD-1); (iii) 1.26 pmol cm⁻² RGD (KRSR/RGD-2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48-780.28 N mm), bone-to-implant contact (BIC) (35.22%-41.49%), and new bone fill (28.58%-30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL-g-PEG polymer does not increase BIC, bone fill, or interfacial shear strength.
通过表面固定多种短肽序列来增强骨整合，这些短肽序列模拟细胞外基质（ECM）蛋白，如精氨酸-甘氨酸-天冬氨酸（RGD）和赖氨酸-精氨酸-丝氨酸-精氨酸（KRSR），尚未得到广泛探索。此外，化学改性喷砂酸蚀（modSLA）表面的生物功能化的效果尚不清楚。本研究评估了用 RGD 和 KRSR 修饰的 modSLA 植入物表面，以潜在增强骨再生早期阶段的骨附着和界面剪切强度。两组实验性植入物分别植入 8 头小型猪的上颌骨中，这些猪的伤口愈合动力学很快：一侧为骨室植入物，形成两个圆形骨缺损，用于组织形态计量学分析；另一侧为标准螺纹构型植入物，用于去除扭矩测试。使用聚-L-赖氨酸-接枝-聚（乙二醇）（PLL-g-PEG）作为载体最小化非特异性蛋白吸附，用三种不同的生物功能化 modSLA 表面[（i）单独 20 pmol cm⁻² KRSR（KRSR）；或与两种不同浓度的 RGD 组合；（ii）0.05 pmol cm⁻² RGD（KRSR/RGD-1）；（iii）1.26 pmol cm⁻² RGD（KRSR/RGD-2）]进行比较，并与（iv）对照 modSLA 进行比较。动物在 2 周时处死。去除扭矩值（701.48-780.28 N mm）、骨与植入物接触（BIC）（35.22%-41.49%）和新骨填充（28.58%-30.62%）在治疗组之间没有显著差异。可以得出结论，用 PLL-g-PEG 聚合物的 KRSR 和 RGD 衍生物生物功能化 modSLA 表面不会增加 BIC、骨填充或界面剪切强度。
