School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China.
School of Traditional Chinese Materia Medical, Shenyang Pharmaceutical University, Shenyang 110016, China.
Molecules. 2022 Jan 20;27(3):678. doi: 10.3390/molecules27030678.
leaves have been widely used for the treatment of inflammation, diabetes, high blood pressure, and other diseases, due to being rich in polyphenols. The main objective of this work was to largely separate the main polyphenols from leaves using the technique of high-speed counter-current chromatography (HSCCC). The phenolic composition in leaves was first analyzed qualitatively and quantitatively by UPLC-Q-Exactive Orbitrap/MS and UPLC-QqQ/MS, respectively, indicating that quercetin and kaempferol derivatives, phenolic acid and apigenin are the main polyphenols in leaves, with quercetin and kaempferol derivatives predominating. Furthermore, the conditions of HSCCC for large-scale separation of polyphenols from leaves were optimized, which included the selection of the solvent system, flow rate and the sample load. Only by one-step HSCCC separation (within 120 min) under the optimized conditions, six quercetin and kaempferol derivatives, a phenolic acid and an apigenin could be individually isolated at a large scale (yield from 10% to 98%), each of which possessed high purity. Finally, the isolated polyphenols and phenolic extract from leaves (MLPE) were verified to have strong neuroprotective activities against HO-induced oxidative stress in PC-12 cells, suggesting that these compounds would contribute to the main beneficial effects of leaves.
叶子因富含多酚而被广泛用于治疗炎症、糖尿病、高血压和其他疾病。本工作的主要目的是使用高速逆流色谱（HSCCC）技术从叶子中大量分离主要的多酚。通过 UPLC-Q-Exactive Orbitrap/MS 和 UPLC-QqQ/MS 分别对 叶子中的酚类成分进行定性和定量分析，表明槲皮素和山柰酚衍生物、酚酸和芹菜素是 叶子中的主要多酚，其中槲皮素和山柰酚衍生物占主导地位。此外，还优化了 HSCCC 从 叶子中大规模分离多酚的条件，包括溶剂系统、流速和样品负载的选择。仅通过在优化条件下的一步 HSCCC 分离（120 分钟内），就可以分别大规模地（产率为 10%至 98%）单独分离出六种槲皮素和山柰酚衍生物、一种酚酸和一种芹菜素，它们都具有很高的纯度。最后，验证了从 叶子中分离得到的多酚和酚提取物（MLPE）对 HO 诱导的 PC-12 细胞氧化应激具有很强的神经保护活性，表明这些化合物将有助于 叶子的主要有益作用。
