King Saud University, Riyadh, Saudi Arabia.
Cell Immunol. 2012;273(1):10-6. doi: 10.1016/j.cellimm.2011.12.003. Epub 2011 Dec 17.
In the present study we evaluated the anti-tumor potential of samsum ant venom (SAV) from Pachycondyla sennaarensis on the human breast carcinoma cell line MCF-7. We found that SAV induced growth arrest of MCF-7 cells without affecting the viability of MCF-10 (non-tumorigenic normal breast epithelial cells) and normal PBMCs. We then analyzed its impact on IGF-1-mediated MCF-7 cell proliferation and its effect on the underlying IGF-1 signaling pathways. Using flow cytometry analysis, we showed that the percentage of apoptotic cells was fourfold higher in SAV-treated cells as compared to untreated cells. More importantly, treatment with SAV induced a marked reduction in actin polymerization and a subsequent marked reduction in IGF-1-mediated cell proliferation. In addition to growth-inhibitory and pro-apoptotic effects, significant reductions were also observed in the phosphorylation of AKT and ERK, but not p38MAPK, in SAV-treated cells as compared to untreated cells. Our data reveal unique anti-tumor effects of samsum ant venom.
在本研究中，我们评估了来自 Pachycondyla sennaarensis 的 samsum 蚁毒液（SAV）对人乳腺癌细胞系 MCF-7 的抗肿瘤潜力。我们发现 SAV 诱导 MCF-7 细胞生长停滞，而不影响 MCF-10（非致瘤性正常乳腺上皮细胞）和正常 PBMCs 的活力。然后，我们分析了它对 IGF-1 介导的 MCF-7 细胞增殖的影响及其对潜在 IGF-1 信号通路的影响。通过流式细胞术分析，我们显示与未处理的细胞相比，用 SAV 处理的细胞中凋亡细胞的百分比高四倍。更重要的是，SAV 处理诱导肌动蛋白聚合明显减少，随后 IGF-1 介导的细胞增殖明显减少。与未处理的细胞相比，SAV 处理的细胞中 AKT 和 ERK 的磷酸化显著减少，但 p38MAPK 没有减少。我们的数据揭示了 samsum 蚁毒液的独特抗肿瘤作用。
