Miyamoto Shinji, Shinmyouzu Kouhei, Miyamoto Ikuya, Takeshita Kenji, Terada Toshihisa, Takahashi Tetsu
Division of Oral and Maxillofacial Reconstructive Surgery, Department of Oral and Maxillofacial Surgery, Science of Physical Functions, Kyushu Dental College, Kitakyusyu City, Japan.
Clin Oral Implants Res. 2013 Aug;24 Suppl A100:134-8. doi: 10.1111/j.1600-0501.2011.02396.x. Epub 2012 Jan 6.
This study utilized the constitution and expression of Runx2/Cbfa1 to conduct 6-month-post-operation histomorphometrical and histochemical analysis of osteocalcin in bone regeneration following sinus-floor augmentation procedures using β-tricalcium phosphate (β-TCP) and autogenous cortical bone.
Thirteen sinuses of nine patients were treated with sinus-floor augmentation using 50% β-TCP and 50% autogenous cancellous bone harvested from the ramus of the mandible. Biopsies of augmented sinuses were taken at 6 months for histomorphometric and immunohistochemical measurements.
Runx2/Cbfa1- and osteocalcin-positive cells were found around TCP particles and on the bone surface. Approximately 60% of cells found around TCP particles stained positive for Runx2/Cbfa1. Fewer cells stained positive for osteocalcin. These positive cells decreased apically with increasing vertical distance from the maxillary bone surface. Histomorphometric analysis showed that the augmented site close to residual bone and periosteum contained approximately 42% bony tissue and 42% soft connective tissue, and the remaining 16% consisted of TCP particles. On the other hand, the augmented bone far from residual bone and periosteum contained 35% bony tissue and 50% soft connective tissue.
Our data suggest that TCP particles attract osteoprogenitor cells that migrate into the interconnecting micropores of the bone-substitute material by 6 months. The augmented site close to residual bone contained a higher proportion of bony tissue and a lower proportion of soft connective tissue than did the augmented site far from residual bone.
本研究利用Runx2/Cbfa1的构成和表达，对使用β-磷酸三钙（β-TCP）和自体皮质骨进行上颌窦底提升术后6个月骨再生过程中的骨钙素进行组织形态计量学和组织化学分析。
9例患者的13个上颌窦采用50%β-TCP和50%取自下颌升支的自体松质骨进行上颌窦底提升治疗。在6个月时对提升后的上颌窦进行活检，进行组织形态计量学和免疫组织化学测量。
在TCP颗粒周围和骨表面发现了Runx2/Cbfa1和骨钙素阳性细胞。在TCP颗粒周围发现的细胞中，约60%Runx2/Cbfa1染色呈阳性。骨钙素染色呈阳性的细胞较少。随着距上颌骨表面垂直距离的增加，这些阳性细胞在根尖方向减少。组织形态计量学分析显示，靠近残余骨和骨膜的提升部位约含42%的骨组织和42%的软结缔组织，其余16%为TCP颗粒。另一方面，远离残余骨和骨膜的提升骨含35%的骨组织和50%的软结缔组织。
我们的数据表明，到6个月时，TCP颗粒吸引骨祖细胞迁移到骨替代材料的相互连通微孔中。靠近残余骨的提升部位比远离残余骨的提升部位含有更高比例的骨组织和更低比例的软结缔组织。
