Liu Xiu-Xiu, Jian Xi-Xian, Cai Xiu-Feng, Chao Ruo-Bing, Chen Qiao-Hong, Chen Dong-Lin, Wang Xiao-Liang, Wang Feng-Peng
Department of Medicinal Analysis, West China College of Pharmacy, Sichuan University, Chengdu 610041, P R China.
Chem Pharm Bull (Tokyo). 2012;60(1):144-9. doi: 10.1248/cpb.60.144.
Bioassay-guided fractionation of an n-BuOH extract of the lateral roots of Aconitum carmichaeli. led to the isolation of 5 cardioactive C(19)-diterpenoid alkaloids: N-deethylaconine (1), beiwutinine (2), hypaconine (3), mesaconine (4), and 15α-hydroxyneoline (5). N-Deethylaconine and beiwutinine are new aconitine-type C(19)-diterpenoid alkaloids. Hypaconine was isolated from this species for the first time. Among them, mesaconine, hypaconine, and beiwutinine showed the strongest cardiac actions on the isolated perfused bullfrog heart. Furthermore, mesaconine has protective effects, including improved inotropic effect and left ventricular diastolic function, on myocardial ischemia-reperfusion injury in rat at a dose of 10(-9) mol/L. However, mesaconine has almost no effect on heart rate.
对乌头侧根正丁醇提取物进行生物活性导向分离，得到了5种具有心脏活性的C(19)-二萜生物碱：去甲乌药碱（1）、北乌头碱（2）、次乌头碱（3）、中乌头碱（4）和15α-羟基新乌头碱（5）。去甲乌药碱和北乌头碱是新的乌头碱型C(19)-二萜生物碱。次乌头碱首次从该物种中分离得到。其中，中乌头碱、次乌头碱和北乌头碱对离体灌注牛蛙心脏显示出最强的心脏作用。此外，中乌头碱在10(-9)mol/L剂量下对大鼠心肌缺血再灌注损伤具有保护作用，包括改善心肌收缩力和左心室舒张功能。然而，中乌头碱对心率几乎没有影响。
