Department of Physiology, Faculty of Pharmacy and the Nutrition and Food Safety Research Institute of the Universitat de Barcelona, Barcelona, Spain.
J Nutr. 2012 Feb;142(2):264-70. doi: 10.3945/jn.111.149070. Epub 2012 Jan 5.
We examined the effects of oral plasma protein supplements on the pulmonary adaptive immune response in mice challenged with intranasal LPS. C57BL/6 mice were fed a control diet or a diet supplemented with plasma proteins [spray-dried plasma (SDP) 80 g/kg] or with an Ig concentrate [(IC) 20 g/kg] from postnatal d 19 (weaning) until d 34. Mice were challenged with PBS or LPS from Escherichia coli at d 33 and killed 24 h later for leukocyte analyses or at d 34 and killed 6 h later for cytokine determination. LPS induced the activation of T helper (Th) lymphocytes in lung and blood and this response was reduced by SDP and IC (P < 0.05). In both tissues, LPS increased the Th1 and Th2 subpopulations and this effect was inhibited by the two plasma protein supplements (P < 0.05). The LPS challenge increased the expression of all the cytokines studied (P < 0.01). SDP and IC reduced the expression of IFNγ, IL-5, IL-12p40, IL-12p70, IL-13, and IL-17 in both tissues, whereas they increased the percentage of regulatory Th lymphocytes in lung, even in PBS-treated mice (P < 0.05). LPS reduced the concentration of mature TGFβ1 (P < 0.05) in the lung but did not modify the expression of IL-10. Mice exposed to LPS and supplemented with SDP or IC showed an increased expression of the anti-inflammatory cytokine IL-10 (P < 0.05). Moreover, the two supplements increased the concentration of IL-10 in intestinal mucosa (P < 0.05). Our results show that plasma supplementation reduces the immune response that characterizes the acute lung inflammation syndrome.
我们研究了口服血浆蛋白补充剂对经鼻腔 LPS 攻击的小鼠肺部适应性免疫反应的影响。C57BL/6 小鼠从出生后第 19 天（断奶）到第 34 天，分别喂食对照饮食或添加血浆蛋白的饮食[喷雾干燥血浆（SDP）80 g/kg]或免疫球蛋白浓缩物[（IC）20 g/kg]。第 33 天，小鼠用 PBS 或大肠杆菌 LPS 进行攻毒，并在 24 小时后处死进行白细胞分析，或在第 34 天处死并在 6 小时后进行细胞因子测定。LPS 诱导肺部和血液中的辅助性 T 淋巴细胞（Th）激活，而 SDP 和 IC 可降低这种反应（P<0.05）。在两种组织中，LPS 增加了 Th1 和 Th2 亚群，而这两种血浆蛋白补充剂均抑制了这种作用（P<0.05）。LPS 攻击增加了所有研究细胞因子的表达（P<0.01）。SDP 和 IC 降低了两种组织中 IFNγ、IL-5、IL-12p40、IL-12p70、IL-13 和 IL-17 的表达，而在 PBS 处理的小鼠中甚至增加了肺部调节性 Th 淋巴细胞的比例（P<0.05）。LPS 降低了肺部成熟 TGFβ1 的浓度（P<0.05），但不改变 IL-10 的表达。暴露于 LPS 并补充 SDP 或 IC 的小鼠显示抗炎细胞因子 IL-10 的表达增加（P<0.05）。此外，两种补充剂增加了肠道黏膜中 IL-10 的浓度（P<0.05）。我们的结果表明，血浆补充剂可降低急性肺炎症候群的免疫反应。
