犬内脏利什曼病临床特征相关抗利什曼原虫抗体谱。
Profile of anti-Leishmania antibodies related to clinical picture in canine visceral leishmaniasis.
机构信息
Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Estadual do Ceará (UECE), Avenida Paranjana, 1700, Campus do Itaperi, Serrinha, CEP 60740-000, Fortaleza, CE, Brazil.
出版信息
Res Vet Sci. 2012 Oct;93(2):705-9. doi: 10.1016/j.rvsc.2011.12.009. Epub 2012 Jan 5.
This research investigated the profile of anti-Leishmania antibodies in different clinical forms of canine visceral leishmaniasis (CVL). Naturally infected dogs were divided into two groups: subclinical dogs (SD, n=10) and clinical dogs (CD, n=68). Non-infected dogs (ND, n=7) comprised the negative control group. The humoral response was evaluated by the profile of total IgG, IgG1, IgG2, IgM, IgA and IgE, determined by ELISA. Infected animals showed increased levels of total IgG, IgA and IgE in addition to IgG1 and IgG2 in groups SD and CD, when compared with group ND. Furthermore, it was observed that IgG2 and IgM were correlated with symptomatology, while total IgG, IgG1 and IgA were negatively correlated and IgE showed no correlation. It follows that serum levels of IgG2 anti-Leishmania are correlated with typical clinical signs of disease. Furthermore the determination of specific anti-Leishmania antibodies could be an important tool in monitoring CVL clinical picture.
本研究调查了不同临床形式的犬内脏利什曼病（CVL）中抗利什曼原虫抗体的特征。自然感染的狗被分为两组：亚临床犬（SD，n=10）和临床犬（CD，n=68）。未感染的狗（ND，n=7）组成阴性对照组。通过 ELISA 测定总 IgG、IgG1、IgG2、IgM、IgA 和 IgE 的图谱来评估体液反应。与 ND 组相比，SD 和 CD 组感染动物的总 IgG、IgA 和 IgE 水平升高，同时 IgG1 和 IgG2 也升高。此外，还观察到 IgG2 和 IgM 与症状相关，而总 IgG、IgG1 和 IgA 呈负相关，IgE 则无相关性。因此，血清 IgG2 抗利什曼原虫水平与疾病的典型临床症状相关。此外，特异性抗利什曼原虫抗体的测定可能是监测 CVL 临床特征的重要工具。
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