Department of Pediatrics, Bucheon St. Mary's Hospital, The Catholic University of Korea, Korea.
Int J Lab Hematol. 2012 Jun;34(3):283-9. doi: 10.1111/j.1751-553X.2011.01392.x. Epub 2012 Jan 9.
The utility of WBC cell population data (CPD) for the differential diagnosis of viral infection from normal control, bacterial infection, and tuberculosis in children was investigated.
A data set of 602 total whole-blood samples were analyzed on the DxH 800 System for complete blood cell count (CBC) with leukocyte differential from children with the following sample breakdown: 77 confirmed diagnoses of viral infections (Epstein-Barr virus; 30, influenza A; 19, rota virus; 11, other viruses;17), 54 normal control, 71 bacterial infection, 17 TB patients, and 383 with various diseases. The mean (MN) and standard deviation (SD) of the volume (V), conductivity (C), five light-scatter measurements, and 14 calculated parameters were obtained for the leukocytes.
Using a combination of the CBC and CPD parameter values, a decision rule, composed of 21 parameters, for the screening of viral infection in children was developed. Using this decision rule, 74 of 77 (96.1%) viral infections, two of 54 (3.7%) normal samples, one of 17 (5.9%) TB, and six of 71 (8.5%) bacterial infection samples were identified. The sensitivity was 96.1%, and specificity for normal control was 96.3% with an overall specificity of 93.7%. Fifty-nine samples of 383 samples (15.4%) collected from in-patient children with various diseases without confirmation of viral infection were included in this decision rule.
In conclusion, the implementation of leukocytes CPD parameters can be useful in the detection of viral infection in children.
本研究旨在探讨白细胞细胞群体数据（CPD）在儿童病毒感染、正常对照、细菌感染和结核病鉴别诊断中的应用价值。
我们分析了 602 份来自儿童的全血样本，这些样本在 DxH 800 系统上进行了完整的血细胞计数（CBC）和白细胞分类，样本分类如下：77 例确诊的病毒感染（EBV；30 例，流感 A；19 例，轮状病毒；11 例，其他病毒；17 例）、54 例正常对照、71 例细菌感染、17 例结核病患者和 383 例其他疾病患者。我们获得了白细胞的体积（V）、传导率（C）、五个光散射测量值和 14 个计算参数的均值（MN）和标准差（SD）。
使用 CBC 和 CPD 参数值的组合，我们为儿童病毒感染的筛查制定了一个由 21 个参数组成的决策规则。使用该决策规则，77 例病毒感染中的 74 例（96.1%）、54 例正常样本中的 2 例（3.7%）、17 例结核病中的 1 例（5.9%）和 71 例细菌感染中的 6 例（8.5%）被正确识别。该方法的敏感性为 96.1%，对正常对照的特异性为 96.3%，总体特异性为 93.7%。在该决策规则中还纳入了 383 例患有各种疾病且未经病毒感染确认的住院患儿的 59 份样本（15.4%）。
总之，白细胞 CPD 参数的应用有助于儿童病毒感染的检测。
