胚胎细胞的培养与操作
Culture and manipulation of embryonic cells.
作者信息
Edgar Lois G, Goldstein Bob
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Colorado, USA.
出版信息
Methods Cell Biol. 2012;107:151-75. doi: 10.1016/B978-0-12-394620-1.00005-9.
Abstract
The direct manipulation of embryonic cells is an important tool for addressing key questions in cell and developmental biology. C. elegans is relatively unique among genetic model systems in being amenable to manipulation of embryonic cells. Embryonic cell manipulation has allowed the identification of cell interactions by direct means, and it has been an important technique for dissecting mechanisms by which cell fates are specified, cell divisions are oriented, and morphogenesis is accomplished. Here, we present detailed methods for isolating, manipulating and culturing embryonic cells of C. elegans.
摘要
直接操控胚胎细胞是解决细胞与发育生物学关键问题的一项重要工具。秀丽隐杆线虫在遗传模型系统中相对独特，因为它易于对胚胎细胞进行操控。胚胎细胞操控使得通过直接手段识别细胞间相互作用成为可能，并且它一直是剖析细胞命运如何被确定、细胞分裂如何定向以及形态发生如何完成的机制的一项重要技术。在此，我们展示了分离、操控和培养秀丽隐杆线虫胚胎细胞的详细方法。
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