Su Yangqi, Shea Jonathan, Destephanis Darla, Su Zhengchang
Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, Charlotte, NC, United States.
Front Cell Dev Biol. 2024 Aug 19;12:1436975. doi: 10.3389/fcell.2024.1436975. eCollection 2024.
hermaphrodite presents a unique model to study the formation of oocytes. However, the size of the model animal and difficulties in retrieval of specific stages of the germline have obviated closer systematic studies of this process throughout the years. Here, we present a transcriptomic level analysis into the oogenesis of hermaphrodites. We dissected a hermaphrodite gonad into seven sections corresponding to the mitotic distal region, the pachytene region, the diplotene region, the early diakinesis region and the 3 most proximal oocytes, and deeply sequenced the transcriptome of each of them along with that of the fertilized egg using a single-cell RNA-seq (scRNA-seq) protocol. We identified specific gene expression events as well as gene splicing events in finer detail along the gonad and provided novel insights into underlying mechanisms of the oogenesis process. Furthermore, through careful review of relevant research literature coupled with patterns observed in our analysis, we delineate transcripts that may serve functions in the interactions between the germline and cells of the somatic gonad. These results expand our knowledge of the transcriptomic space of the germline and lay a foundation on which future studies of the germline can be based upon.
雌雄同体动物为研究卵母细胞的形成提供了一个独特的模型。然而，多年来，模型动物的体型以及获取生殖系特定阶段细胞的困难阻碍了对这一过程进行更深入的系统研究。在此，我们对雌雄同体动物的卵子发生进行了转录组水平的分析。我们将雌雄同体动物的性腺解剖成七个部分，分别对应有丝分裂远端区域、粗线期区域、双线期区域、早终变期区域以及最靠近近端的3个卵母细胞，并使用单细胞RNA测序（scRNA-seq）方案对每个部分以及受精卵的转录组进行了深度测序。我们更详细地确定了沿性腺的特定基因表达事件以及基因剪接事件，并为卵子发生过程的潜在机制提供了新的见解。此外，通过仔细回顾相关研究文献并结合我们分析中观察到的模式，我们描绘了可能在生殖系与体细胞性腺细胞之间的相互作用中发挥作用的转录本。这些结果扩展了我们对生殖系转录组空间的认识，并为未来生殖系研究奠定了基础。
