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本文引用的文献

1
alpha-Hydroxynitrile lyase protein from Xylella fastidiosa: Cloning, expression, and characterization.来自桑氏假单胞菌的α-羟基腈裂合酶蛋白：克隆、表达及特性分析
Microb Pathog. 2009 Sep;47(3):118-27. doi: 10.1016/j.micpath.2009.06.007. Epub 2009 Jul 1.
2
Structure-based phylogeny as a diagnostic for functional characterization of proteins with a cupin fold.基于结构的系统发育分析作为一种用于具有cupin折叠的蛋白质功能表征的诊断方法。
PLoS One. 2009 May 29;4(5):e5736. doi: 10.1371/journal.pone.0005736.
3
Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity.杏仁中依赖黄素腺嘌呤二核苷酸的醇腈酶的底物结合为氰醇形成机制提供了见解，并解释了脱氢活性的缺失。
Biochemistry. 2009 Apr 21;48(15):3370-7. doi: 10.1021/bi802162s.
4
Cyanogenesis in plants and arthropods.植物和节肢动物中的氰化物生成
Phytochemistry. 2008 May;69(7):1457-68. doi: 10.1016/j.phytochem.2008.02.019. Epub 2008 Mar 18.
5
Bacterial endophytes: recent developments and applications.细菌内生菌：最新进展与应用
FEMS Microbiol Lett. 2008 Jan;278(1):1-9. doi: 10.1111/j.1574-6968.2007.00918.x. Epub 2007 Nov 21.
6
An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.一种用于在大肠杆菌中对大量PCR片段进行表达克隆的高效质粒载体。
Appl Microbiol Biotechnol. 2007 Nov;77(1):241-4. doi: 10.1007/s00253-007-1151-1. Epub 2007 Sep 5.
7
Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry.醇腈酶作为化学工业中生物催化剂的潜力和能力。
Appl Microbiol Biotechnol. 2007 Aug;76(2):309-20. doi: 10.1007/s00253-007-1025-6. Epub 2007 Jul 3.
8
A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening.一种适用于高通量筛选的新型羟基腈裂解酶筛选测定法。
J Biotechnol. 2007 Mar 30;129(1):151-61. doi: 10.1016/j.jbiotec.2006.10.004. Epub 2006 Oct 14.
9
Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities.田间种植马铃薯植株的内生细菌群落及其促植物生长和拮抗能力。
Can J Microbiol. 2004 Apr;50(4):239-49. doi: 10.1139/w03-118.
10
Potential role of pathogen signaling in multitrophic plant-microbe interactions involved in disease protection.病原体信号在参与疾病保护的多营养级植物-微生物相互作用中的潜在作用。
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两种与 cupin 超家族蛋白具有高度相似性的细菌羟腈裂解酶的特性。

Characterization of two bacterial hydroxynitrile lyases with high similarity to cupin superfamily proteins.

机构信息

Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria.

出版信息

Appl Environ Microbiol. 2012 Mar;78(6):2053-5. doi: 10.1128/AEM.06899-11. Epub 2012 Jan 6.

DOI:10.1128/AEM.06899-11
PMID:22226952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3298160/
Abstract

Hydroxynitrile lyases (HNLs) catalyze the cleavage of cyanohydrins. In the reverse reaction, they catalyze the formation of carbon-carbon bonds by enantioselective condensation of hydrocyanic acid with carbonyls. In this study, we describe two proteins from endophytic bacteria that display activity in the cleavage and the synthesis reaction of (R)-mandelonitrile with up to 74% conversion of benzaldehyde (enantiopreference ee 89%). Both showed high similarity to proteins of the cupin superfamily which so far were not known to exhibit HNL activity.

摘要

羟腈裂解酶（HNLs）催化氰醇的裂解。在逆反应中，它们通过氰化氢与羰基的对映选择性缩合催化碳-碳键的形成。在这项研究中，我们描述了两种来自内生细菌的蛋白质，它们在（R）-扁桃腈的裂解和合成反应中表现出活性，苯甲醛的转化率高达 74%（对映体偏好 ee89%）。这两种蛋白质都与杯状蛋白超家族的蛋白质具有高度相似性，而这些蛋白质迄今尚未被发现具有 HNL 活性。