Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 South Grand Boulevard, Saint Louis, MO 63104, United States.
Peptides. 2012 Feb;33(2):342-5. doi: 10.1016/j.peptides.2011.12.013. Epub 2011 Dec 31.
We recently reported that neuronostatin, a novel neuropeptide, biphasically increased mean arterial pressure, first through the activation of the sympathetic nervous system followed by the release of vasopressin. In those experiments, we found that centrally administered neuronostatin increased plasma vasopressin levels only 2-3 times greater than levels observed in saline-treated controls, and that the increase in mean arterial pressure (approximately 15 mm Hg) could be blocked by pretreatment with a V1-vasopressin antagonist. Here we report the relationship between two to three fold elevations in plasma vasopressin levels and concomitant changes in mean arterial pressure in conscious, unrestrained male rats. We injected increasing doses of vasopressin (5, 20, and 100 ng/kg, intra-arterially) and measured both changes in plasma vasopressin levels and the elevation in mean arterial pressure achieved. At 5-min post injection, plasma levels of vasopressin and mean arterial pressures were similar to those observed following central neuronostatin administration in our earlier study. Thus we conclude that small increases in circulating vasopressin levels can result in significant elevations in mean arterial pressure at least in the conscious rat.
我们最近报道了一种新的神经肽——神经降压素，它能呈双相性地增加平均动脉压，首先通过激活交感神经系统，然后释放血管加压素。在那些实验中，我们发现中枢给予神经降压素后，血浆血管加压素水平仅升高 2-3 倍，高于生理盐水处理对照组观察到的水平，而平均动脉压的升高（约 15 毫米汞柱）可通过预先给予 V1 血管加压素拮抗剂来阻断。在这里，我们报告了在清醒、不受约束的雄性大鼠中，血浆血管加压素水平升高 2-3 倍与平均动脉压变化之间的关系。我们注射了递增剂量的血管加压素（5、20 和 100ng/kg，动脉内），并测量了血浆血管加压素水平的变化和平均动脉压的升高。在注射后 5 分钟，血浆血管加压素水平和平均动脉压与我们早期研究中中枢给予神经降压素后观察到的水平相似。因此，我们得出结论，循环中血管加压素水平的微小增加至少可导致清醒大鼠的平均动脉压显著升高。
