Department of Chemical Engineering, Columbia University in the City of New York, New York, NY 10027, USA.
Chem Commun (Camb). 2012 Feb 11;48(13):1898-900. doi: 10.1039/c2cc16156g. Epub 2012 Jan 6.
The performance of immobilized enzyme systems is often limited by cofactor diffusion and regeneration. Here, we demonstrate an engineered enzyme capable of utilizing the minimal cofactor nicotinamide mononucleotide (NMN(+)) to address these limitations. Significant gains in performance are observed with NMN(+) in immobilized systems, despite a decreased turnover rate with the minimal cofactor.
固定化酶系统的性能通常受到辅因子扩散和再生的限制。在这里，我们展示了一种经过工程改造的酶，它能够利用最小的辅因子烟酰胺单核苷酸（NMN(+))来解决这些限制。尽管最小辅因子的周转率降低，但在固定化系统中使用 NMN(+) 时，性能有了显著提高。
