Apushkinskaya N, Zolotukhina E V, Butyrskaya E V, Silina Y E
Institute of Biochemistry, Saarland University, 66123, Saarbrücken, Campus B 2.2, Germany.
Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Moscow Region, Russia.
Comput Struct Biotechnol J. 2022 Jul 12;20:3824-3832. doi: 10.1016/j.csbj.2022.07.012. eCollection 2022.
During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the substrates to the products. Herein, we show how the immobilization of the required electroplated cofactors in a design of amperometric electrodes can assist the activity of apo-enzymes. Compared to conventional approaches used in enzyme engineering this tailored nanoengineering methodology is superior from economic point of view, labor and time costs, storage conditions, reduced amount of waste and can fill the gap in the development of tuned bioelectrocatalysts.
在产物分离过程中，由于多步解离和辅因子的损失，所获得的生物受体往往不表现出足够的生化活性。然而，对于生物电化学应用而言，辅因子的存在对于将底物成功氧化或还原转化为产物是必要的。在此，我们展示了如何在安培电极设计中固定所需的电镀辅因子，以协助脱辅基酶的活性。与酶工程中使用的传统方法相比，这种定制的纳米工程方法在经济、劳动力和时间成本、储存条件、减少废物量方面具有优势，并且可以填补定制生物电催化剂开发中的空白。
